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Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation

BACKGROUND: An outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Jordan in 2015 involved a variant virus that acquired distinctive deletions in the accessory open reading frames. We conducted a molecular and seroepidemiologic investigation to describe the deletion variant’s tran...

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Autores principales: Payne, Daniel C, Biggs, Holly M, Al-Abdallat, Mohammad Mousa, Alqasrawi, Sultan, Lu, Xiaoyan, Abedi, Glen R, Haddadin, Aktham, Iblan, Ibrahim, Alsanouri, Tarek, Al Nsour, Mohannad, Sheikh Ali, Sami, Rha, Brian, Trivedi, Suvang U, Rasheed, Mohammed Ata Ur, Tamin, Azaibi, Lamers, Mart M, Haagmans, Bart L, Erdman, Dean D, Thornburg, Natalie J, Gerber, Susan I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965092/
https://www.ncbi.nlm.nih.gov/pubmed/30294616
http://dx.doi.org/10.1093/ofid/ofy095
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author Payne, Daniel C
Biggs, Holly M
Al-Abdallat, Mohammad Mousa
Alqasrawi, Sultan
Lu, Xiaoyan
Abedi, Glen R
Haddadin, Aktham
Iblan, Ibrahim
Alsanouri, Tarek
Al Nsour, Mohannad
Sheikh Ali, Sami
Rha, Brian
Trivedi, Suvang U
Rasheed, Mohammed Ata Ur
Tamin, Azaibi
Lamers, Mart M
Haagmans, Bart L
Erdman, Dean D
Thornburg, Natalie J
Gerber, Susan I
author_facet Payne, Daniel C
Biggs, Holly M
Al-Abdallat, Mohammad Mousa
Alqasrawi, Sultan
Lu, Xiaoyan
Abedi, Glen R
Haddadin, Aktham
Iblan, Ibrahim
Alsanouri, Tarek
Al Nsour, Mohannad
Sheikh Ali, Sami
Rha, Brian
Trivedi, Suvang U
Rasheed, Mohammed Ata Ur
Tamin, Azaibi
Lamers, Mart M
Haagmans, Bart L
Erdman, Dean D
Thornburg, Natalie J
Gerber, Susan I
author_sort Payne, Daniel C
collection PubMed
description BACKGROUND: An outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Jordan in 2015 involved a variant virus that acquired distinctive deletions in the accessory open reading frames. We conducted a molecular and seroepidemiologic investigation to describe the deletion variant’s transmission patterns and epidemiology. METHODS: We reviewed epidemiologic and medical chart data and analyzed viral genome sequences from respiratory specimens of MERS-CoV cases. In early 2016, sera and standardized interviews were obtained from MERS-CoV cases and their contacts. Sera were evaluated by nucleocapsid and spike protein enzyme immunoassays and microneutralization. RESULTS: Among 16 cases, 11 (69%) had health care exposure and 5 (31%) were relatives of a known case; 13 (81%) were symptomatic, and 7 (44%) died. Genome sequencing of MERS-CoV from 13 cases revealed 3 transmissible deletions associated with clinical illness during the outbreak. Deletion variant sequences were epidemiologically clustered and linked to a common transmission chain. Interviews and sera were collected from 2 surviving cases, 23 household contacts, and 278 health care contacts; 1 (50%) case, 2 (9%) household contacts, and 3 (1%) health care contacts tested seropositive. CONCLUSIONS: The MERS-CoV deletion variants retained human-to-human transmissibility and caused clinical illness in infected persons despite accumulated mutations. Serology suggested limited transmission beyond that detected during the initial outbreak investigation.
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spelling pubmed-59650922018-10-05 Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation Payne, Daniel C Biggs, Holly M Al-Abdallat, Mohammad Mousa Alqasrawi, Sultan Lu, Xiaoyan Abedi, Glen R Haddadin, Aktham Iblan, Ibrahim Alsanouri, Tarek Al Nsour, Mohannad Sheikh Ali, Sami Rha, Brian Trivedi, Suvang U Rasheed, Mohammed Ata Ur Tamin, Azaibi Lamers, Mart M Haagmans, Bart L Erdman, Dean D Thornburg, Natalie J Gerber, Susan I Open Forum Infect Dis Major Article BACKGROUND: An outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Jordan in 2015 involved a variant virus that acquired distinctive deletions in the accessory open reading frames. We conducted a molecular and seroepidemiologic investigation to describe the deletion variant’s transmission patterns and epidemiology. METHODS: We reviewed epidemiologic and medical chart data and analyzed viral genome sequences from respiratory specimens of MERS-CoV cases. In early 2016, sera and standardized interviews were obtained from MERS-CoV cases and their contacts. Sera were evaluated by nucleocapsid and spike protein enzyme immunoassays and microneutralization. RESULTS: Among 16 cases, 11 (69%) had health care exposure and 5 (31%) were relatives of a known case; 13 (81%) were symptomatic, and 7 (44%) died. Genome sequencing of MERS-CoV from 13 cases revealed 3 transmissible deletions associated with clinical illness during the outbreak. Deletion variant sequences were epidemiologically clustered and linked to a common transmission chain. Interviews and sera were collected from 2 surviving cases, 23 household contacts, and 278 health care contacts; 1 (50%) case, 2 (9%) household contacts, and 3 (1%) health care contacts tested seropositive. CONCLUSIONS: The MERS-CoV deletion variants retained human-to-human transmissibility and caused clinical illness in infected persons despite accumulated mutations. Serology suggested limited transmission beyond that detected during the initial outbreak investigation. Oxford University Press 2018-04-28 /pmc/articles/PMC5965092/ /pubmed/30294616 http://dx.doi.org/10.1093/ofid/ofy095 Text en Published by Oxford University Press on behalf of Infectious Diseases Society of America 2018. This work is written by (a) US Government employee(s) and is in the public domain in the US.
spellingShingle Major Article
Payne, Daniel C
Biggs, Holly M
Al-Abdallat, Mohammad Mousa
Alqasrawi, Sultan
Lu, Xiaoyan
Abedi, Glen R
Haddadin, Aktham
Iblan, Ibrahim
Alsanouri, Tarek
Al Nsour, Mohannad
Sheikh Ali, Sami
Rha, Brian
Trivedi, Suvang U
Rasheed, Mohammed Ata Ur
Tamin, Azaibi
Lamers, Mart M
Haagmans, Bart L
Erdman, Dean D
Thornburg, Natalie J
Gerber, Susan I
Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation
title Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation
title_full Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation
title_fullStr Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation
title_full_unstemmed Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation
title_short Multihospital Outbreak of a Middle East Respiratory Syndrome Coronavirus Deletion Variant, Jordan: A Molecular, Serologic, and Epidemiologic Investigation
title_sort multihospital outbreak of a middle east respiratory syndrome coronavirus deletion variant, jordan: a molecular, serologic, and epidemiologic investigation
topic Major Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965092/
https://www.ncbi.nlm.nih.gov/pubmed/30294616
http://dx.doi.org/10.1093/ofid/ofy095
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