Cargando…
Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optim...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royal Society of Chemistry
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965250/ https://www.ncbi.nlm.nih.gov/pubmed/29899912 http://dx.doi.org/10.1039/c5sc03404c |
_version_ | 1783325327863316480 |
---|---|
author | Tang, Shan Wan, Zhengpeng Gao, Yiren Zheng, Ji-Shen Wang, Jing Si, Yan-Yan Chen, Xin Qi, Hai Liu, Lei Liu, Wanli |
author_facet | Tang, Shan Wan, Zhengpeng Gao, Yiren Zheng, Ji-Shen Wang, Jing Si, Yan-Yan Chen, Xin Qi, Hai Liu, Lei Liu, Wanli |
author_sort | Tang, Shan |
collection | PubMed |
description | We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K(96)NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K(96)NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level. |
format | Online Article Text |
id | pubmed-5965250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-59652502018-06-13 Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions Tang, Shan Wan, Zhengpeng Gao, Yiren Zheng, Ji-Shen Wang, Jing Si, Yan-Yan Chen, Xin Qi, Hai Liu, Lei Liu, Wanli Chem Sci Chemistry We report the chemical synthesis of the first photo-activatable protein antigen that can be used to study antigen–antibody interaction mediated responses in B cells. This strategy facilitated fine tuning of the caged protein antigen to optimize its bioactivity and photochemical properties. One optimal molecule, HEL-K(96)NPE, was totally inert to hen egg lysozyme (HEL)-specific B cells and could only restore its antigenicity upon photoactivation. Combined with real time live cell imaging, the utility of HEL-K(96)NPE was demonstrated as a proof of concept to quantify B cell synapse formation and calcium influx responses at the single cell level. Royal Society of Chemistry 2016-03-01 2015-12-11 /pmc/articles/PMC5965250/ /pubmed/29899912 http://dx.doi.org/10.1039/c5sc03404c Text en This journal is © The Royal Society of Chemistry 2016 http://creativecommons.org/licenses/by/3.0/ This article is freely available. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence (CC BY 3.0) |
spellingShingle | Chemistry Tang, Shan Wan, Zhengpeng Gao, Yiren Zheng, Ji-Shen Wang, Jing Si, Yan-Yan Chen, Xin Qi, Hai Liu, Lei Liu, Wanli Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions |
title | Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
|
title_full | Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
|
title_fullStr | Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
|
title_full_unstemmed | Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
|
title_short | Total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions
|
title_sort | total chemical synthesis of photoactivatable proteins for light-controlled manipulation of antigen–antibody interactions |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965250/ https://www.ncbi.nlm.nih.gov/pubmed/29899912 http://dx.doi.org/10.1039/c5sc03404c |
work_keys_str_mv | AT tangshan totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT wanzhengpeng totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT gaoyiren totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT zhengjishen totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT wangjing totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT siyanyan totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT chenxin totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT qihai totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT liulei totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions AT liuwanli totalchemicalsynthesisofphotoactivatableproteinsforlightcontrolledmanipulationofantigenantibodyinteractions |