Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells

SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strateg...

Descripción completa

Detalles Bibliográficos
Autores principales: Murillo, Jimmy Rodriguez, Goto-Silva, Livia, Sánchez, Aniel, Nogueira, Fábio C.S., Domont, Gilberto B., Junqueira, Magno
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Regular Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965715/
https://www.ncbi.nlm.nih.gov/pubmed/29900121
http://dx.doi.org/10.1016/j.euprot.2017.06.001
_version_ 1783325372503293952
author Murillo, Jimmy Rodriguez
Goto-Silva, Livia
Sánchez, Aniel
Nogueira, Fábio C.S.
Domont, Gilberto B.
Junqueira, Magno
author_facet Murillo, Jimmy Rodriguez
Goto-Silva, Livia
Sánchez, Aniel
Nogueira, Fábio C.S.
Domont, Gilberto B.
Junqueira, Magno
author_sort Murillo, Jimmy Rodriguez
collection PubMed
description SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation.
format Online
Article
Text
id pubmed-5965715
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-59657152018-06-13 Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells Murillo, Jimmy Rodriguez Goto-Silva, Livia Sánchez, Aniel Nogueira, Fábio C.S. Domont, Gilberto B. Junqueira, Magno EuPA Open Proteom Regular Article SH-SY5Y neuroblastoma cells are susceptible to differentiation using retinoic acid (RA) and brain-derived neurotrophic factor (BDNF), providing a model of neuronal differentiation. We compared SH-SY5Y cells proteome before and after RA/BDNF treatment using iTRAQ and phosphopeptide enrichment strategies. We identified 5587 proteins, 366 of them with differential abundance. Differentiated cells expressed proteins related to neuronal development, and, undifferentiated cells expressed proteins involved in cell proliferation. Interactive network covered focal adhesion, cytoskeleton dynamics and neurodegenerative diseases processes and regulation of mitogen-activated protein kinase-related signaling pathways; key proteins involved in those processes might be explored as markers for neuronal differentiation. Elsevier 2017-06-23 /pmc/articles/PMC5965715/ /pubmed/29900121 http://dx.doi.org/10.1016/j.euprot.2017.06.001 Text en © 2017 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Regular Article
Murillo, Jimmy Rodriguez
Goto-Silva, Livia
Sánchez, Aniel
Nogueira, Fábio C.S.
Domont, Gilberto B.
Junqueira, Magno
Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
title Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
title_full Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
title_fullStr Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
title_full_unstemmed Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
title_short Quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated SH-SY5Y neuroblastoma cells
title_sort quantitative proteomic analysis identifies proteins and pathways related to neuronal development in differentiated sh-sy5y neuroblastoma cells
topic Regular Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5965715/
https://www.ncbi.nlm.nih.gov/pubmed/29900121
http://dx.doi.org/10.1016/j.euprot.2017.06.001
work_keys_str_mv AT murillojimmyrodriguez quantitativeproteomicanalysisidentifiesproteinsandpathwaysrelatedtoneuronaldevelopmentindifferentiatedshsy5yneuroblastomacells
AT gotosilvalivia quantitativeproteomicanalysisidentifiesproteinsandpathwaysrelatedtoneuronaldevelopmentindifferentiatedshsy5yneuroblastomacells
AT sanchezaniel quantitativeproteomicanalysisidentifiesproteinsandpathwaysrelatedtoneuronaldevelopmentindifferentiatedshsy5yneuroblastomacells
AT nogueirafabiocs quantitativeproteomicanalysisidentifiesproteinsandpathwaysrelatedtoneuronaldevelopmentindifferentiatedshsy5yneuroblastomacells
AT domontgilbertob quantitativeproteomicanalysisidentifiesproteinsandpathwaysrelatedtoneuronaldevelopmentindifferentiatedshsy5yneuroblastomacells
AT junqueiramagno quantitativeproteomicanalysisidentifiesproteinsandpathwaysrelatedtoneuronaldevelopmentindifferentiatedshsy5yneuroblastomacells

Ejemplares similares