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Reconstitution reveals motor activation for intraflagellar transport
The human body represents a striking example of ciliary diversification. Extending from the surface of most cells, cilia accomplish an astonishingly diverse set of tasks. Predictably, mutations in ciliary genes cause a wide range of human diseases such as male infertility or blindness. In C. elegans...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5967604/ https://www.ncbi.nlm.nih.gov/pubmed/29743676 http://dx.doi.org/10.1038/s41586-018-0105-3 |
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author | Mohamed, Mohamed A. A. Stepp, Willi L. Ökten, Zeynep |
author_facet | Mohamed, Mohamed A. A. Stepp, Willi L. Ökten, Zeynep |
author_sort | Mohamed, Mohamed A. A. |
collection | PubMed |
description | The human body represents a striking example of ciliary diversification. Extending from the surface of most cells, cilia accomplish an astonishingly diverse set of tasks. Predictably, mutations in ciliary genes cause a wide range of human diseases such as male infertility or blindness. In C. elegans sensory cilia, this functional diversity appears to be traceable to the differential regulation of the kinesin-2-powered intraflagellar transport (IFT) machinery. Here, we reconstituted the first functional, multi-component IFT complex that is deployed in the sensory cilia of C. elegans. Our bottom-up approach revealed the molecular basis of specific motor recruitment to the IFT trains. We identified the key component that incorporates homodimeric kinesin-2 into its physiologically relevant context which in turn allosterically activates the motor for efficient transport. These results lay the groundwork for a molecular delineation of IFT regulation that eluded understanding since its ground-breaking discovery more than two decades ago. |
format | Online Article Text |
id | pubmed-5967604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-59676042018-11-09 Reconstitution reveals motor activation for intraflagellar transport Mohamed, Mohamed A. A. Stepp, Willi L. Ökten, Zeynep Nature Article The human body represents a striking example of ciliary diversification. Extending from the surface of most cells, cilia accomplish an astonishingly diverse set of tasks. Predictably, mutations in ciliary genes cause a wide range of human diseases such as male infertility or blindness. In C. elegans sensory cilia, this functional diversity appears to be traceable to the differential regulation of the kinesin-2-powered intraflagellar transport (IFT) machinery. Here, we reconstituted the first functional, multi-component IFT complex that is deployed in the sensory cilia of C. elegans. Our bottom-up approach revealed the molecular basis of specific motor recruitment to the IFT trains. We identified the key component that incorporates homodimeric kinesin-2 into its physiologically relevant context which in turn allosterically activates the motor for efficient transport. These results lay the groundwork for a molecular delineation of IFT regulation that eluded understanding since its ground-breaking discovery more than two decades ago. 2018-05-09 2018-05 /pmc/articles/PMC5967604/ /pubmed/29743676 http://dx.doi.org/10.1038/s41586-018-0105-3 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Mohamed, Mohamed A. A. Stepp, Willi L. Ökten, Zeynep Reconstitution reveals motor activation for intraflagellar transport |
title | Reconstitution reveals motor activation for intraflagellar transport |
title_full | Reconstitution reveals motor activation for intraflagellar transport |
title_fullStr | Reconstitution reveals motor activation for intraflagellar transport |
title_full_unstemmed | Reconstitution reveals motor activation for intraflagellar transport |
title_short | Reconstitution reveals motor activation for intraflagellar transport |
title_sort | reconstitution reveals motor activation for intraflagellar transport |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5967604/ https://www.ncbi.nlm.nih.gov/pubmed/29743676 http://dx.doi.org/10.1038/s41586-018-0105-3 |
work_keys_str_mv | AT mohamedmohamedaa reconstitutionrevealsmotoractivationforintraflagellartransport AT steppwillil reconstitutionrevealsmotoractivationforintraflagellartransport AT oktenzeynep reconstitutionrevealsmotoractivationforintraflagellartransport |