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Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP
The study aimed at investigating the effects of miR-30a-5p on the biological functions of oral cancer cells and figuring out the potential mechanism. We first verified the low expression of miR-30a-5p and high expression of FAP (Homo sapiens fibroblast activation protein α) in oral cancerous tissues...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968186/ https://www.ncbi.nlm.nih.gov/pubmed/29026005 http://dx.doi.org/10.1042/BSR20171027 |
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author | Ruan, Peng Tao, Zezhang Tan, Aili |
author_facet | Ruan, Peng Tao, Zezhang Tan, Aili |
author_sort | Ruan, Peng |
collection | PubMed |
description | The study aimed at investigating the effects of miR-30a-5p on the biological functions of oral cancer cells and figuring out the potential mechanism. We first verified the low expression of miR-30a-5p and high expression of FAP (Homo sapiens fibroblast activation protein α) in oral cancerous tissues and their negative correlation. Then, the target relationship between miR-30a-5p and FAP was validated by dual luciferase reporter assay and biotin-coupled miRNA pulldown assay. After transfection in Tca-8113 cells and SCC-15 cells, MTT, colony formation, Transwell, and wound healing assays were performed to investigate how miR-30a-5p and FAP adjusted propagation, invasiveness, and migration, respectively. Mounting evidence supported that miR-30a-5p directly targetted FAP and suppressed its expression in oral cavity cancer cells (OSCCs). By suppressing FAP expression, miR-30a-5p significantly inhibited cell propagation, migration, and invasion. Therefore, miR-30a-5p might be a new therapeutic target for oral cancer treatment. |
format | Online Article Text |
id | pubmed-5968186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59681862018-06-12 Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP Ruan, Peng Tao, Zezhang Tan, Aili Biosci Rep Research Articles The study aimed at investigating the effects of miR-30a-5p on the biological functions of oral cancer cells and figuring out the potential mechanism. We first verified the low expression of miR-30a-5p and high expression of FAP (Homo sapiens fibroblast activation protein α) in oral cancerous tissues and their negative correlation. Then, the target relationship between miR-30a-5p and FAP was validated by dual luciferase reporter assay and biotin-coupled miRNA pulldown assay. After transfection in Tca-8113 cells and SCC-15 cells, MTT, colony formation, Transwell, and wound healing assays were performed to investigate how miR-30a-5p and FAP adjusted propagation, invasiveness, and migration, respectively. Mounting evidence supported that miR-30a-5p directly targetted FAP and suppressed its expression in oral cavity cancer cells (OSCCs). By suppressing FAP expression, miR-30a-5p significantly inhibited cell propagation, migration, and invasion. Therefore, miR-30a-5p might be a new therapeutic target for oral cancer treatment. Portland Press Ltd. 2018-01-25 /pmc/articles/PMC5968186/ /pubmed/29026005 http://dx.doi.org/10.1042/BSR20171027 Text en © 2018 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Ruan, Peng Tao, Zezhang Tan, Aili Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP |
title | Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP |
title_full | Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP |
title_fullStr | Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP |
title_full_unstemmed | Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP |
title_short | Low expression of miR-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of FAP |
title_sort | low expression of mir-30a-5p induced the proliferation and invasion of oral cancer via promoting the expression of fap |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968186/ https://www.ncbi.nlm.nih.gov/pubmed/29026005 http://dx.doi.org/10.1042/BSR20171027 |
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