Cargando…

Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions

Transcriptomics, the analysis of genome-wide RNA expression, is a common approach to investigate host and pathogen processes in infectious diseases. Technical and bioinformatic advances have permitted increasingly thorough analyses of the association of RNA expression with fundamental biology, immun...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Hyun Jae, Georgiadou, Athina, Otto, Thomas D., Levin, Michael, Coin, Lachlan J., Conway, David J., Cunnington, Aubrey J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968457/
https://www.ncbi.nlm.nih.gov/pubmed/29695497
http://dx.doi.org/10.1128/MMBR.00071-17
_version_ 1783325770645504000
author Lee, Hyun Jae
Georgiadou, Athina
Otto, Thomas D.
Levin, Michael
Coin, Lachlan J.
Conway, David J.
Cunnington, Aubrey J.
author_facet Lee, Hyun Jae
Georgiadou, Athina
Otto, Thomas D.
Levin, Michael
Coin, Lachlan J.
Conway, David J.
Cunnington, Aubrey J.
author_sort Lee, Hyun Jae
collection PubMed
description Transcriptomics, the analysis of genome-wide RNA expression, is a common approach to investigate host and pathogen processes in infectious diseases. Technical and bioinformatic advances have permitted increasingly thorough analyses of the association of RNA expression with fundamental biology, immunity, pathogenesis, diagnosis, and prognosis. Transcriptomic approaches can now be used to realize a previously unattainable goal, the simultaneous study of RNA expression in host and pathogen, in order to better understand their interactions. This exciting prospect is not without challenges, especially as focus moves from interactions in vitro under tightly controlled conditions to tissue- and systems-level interactions in animal models and natural and experimental infections in humans. Here we review the contribution of transcriptomic studies to the understanding of malaria, a parasitic disease which has exerted a major influence on human evolution and continues to cause a huge global burden of disease. We consider malaria a paradigm for the transcriptomic assessment of systemic host-pathogen interactions in humans, because much of the direct host-pathogen interaction occurs within the blood, a readily sampled compartment of the body. We illustrate lessons learned from transcriptomic studies of malaria and how these lessons may guide studies of host-pathogen interactions in other infectious diseases. We propose that the potential of transcriptomic studies to improve the understanding of malaria as a disease remains partly untapped because of limitations in study design rather than as a consequence of technological constraints. Further advances will require the integration of transcriptomic data with analytical approaches from other scientific disciplines, including epidemiology and mathematical modeling.
format Online
Article
Text
id pubmed-5968457
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher American Society for Microbiology
record_format MEDLINE/PubMed
spelling pubmed-59684572018-05-31 Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions Lee, Hyun Jae Georgiadou, Athina Otto, Thomas D. Levin, Michael Coin, Lachlan J. Conway, David J. Cunnington, Aubrey J. Microbiol Mol Biol Rev Review Transcriptomics, the analysis of genome-wide RNA expression, is a common approach to investigate host and pathogen processes in infectious diseases. Technical and bioinformatic advances have permitted increasingly thorough analyses of the association of RNA expression with fundamental biology, immunity, pathogenesis, diagnosis, and prognosis. Transcriptomic approaches can now be used to realize a previously unattainable goal, the simultaneous study of RNA expression in host and pathogen, in order to better understand their interactions. This exciting prospect is not without challenges, especially as focus moves from interactions in vitro under tightly controlled conditions to tissue- and systems-level interactions in animal models and natural and experimental infections in humans. Here we review the contribution of transcriptomic studies to the understanding of malaria, a parasitic disease which has exerted a major influence on human evolution and continues to cause a huge global burden of disease. We consider malaria a paradigm for the transcriptomic assessment of systemic host-pathogen interactions in humans, because much of the direct host-pathogen interaction occurs within the blood, a readily sampled compartment of the body. We illustrate lessons learned from transcriptomic studies of malaria and how these lessons may guide studies of host-pathogen interactions in other infectious diseases. We propose that the potential of transcriptomic studies to improve the understanding of malaria as a disease remains partly untapped because of limitations in study design rather than as a consequence of technological constraints. Further advances will require the integration of transcriptomic data with analytical approaches from other scientific disciplines, including epidemiology and mathematical modeling. American Society for Microbiology 2018-04-25 /pmc/articles/PMC5968457/ /pubmed/29695497 http://dx.doi.org/10.1128/MMBR.00071-17 Text en Copyright © 2018 Lee et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Review
Lee, Hyun Jae
Georgiadou, Athina
Otto, Thomas D.
Levin, Michael
Coin, Lachlan J.
Conway, David J.
Cunnington, Aubrey J.
Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions
title Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions
title_full Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions
title_fullStr Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions
title_full_unstemmed Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions
title_short Transcriptomic Studies of Malaria: a Paradigm for Investigation of Systemic Host-Pathogen Interactions
title_sort transcriptomic studies of malaria: a paradigm for investigation of systemic host-pathogen interactions
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968457/
https://www.ncbi.nlm.nih.gov/pubmed/29695497
http://dx.doi.org/10.1128/MMBR.00071-17
work_keys_str_mv AT leehyunjae transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions
AT georgiadouathina transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions
AT ottothomasd transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions
AT levinmichael transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions
AT coinlachlanj transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions
AT conwaydavidj transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions
AT cunningtonaubreyj transcriptomicstudiesofmalariaaparadigmforinvestigationofsystemichostpathogeninteractions