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Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals

BACKGROUND: Emergence of carbapenem resistant Escherichia coli pathovars and their environmental dissemination are alarming problems. E. coli isolated from sewage water of hospital setting conferred a high resistance towards β-lactams, particularly towards carbapenem. This prompted us to perform who...

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Autores principales: Beg, Ayesha Z., Khan, Asad U.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968484/
https://www.ncbi.nlm.nih.gov/pubmed/29849769
http://dx.doi.org/10.1186/s13099-018-0247-8
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author Beg, Ayesha Z.
Khan, Asad U.
author_facet Beg, Ayesha Z.
Khan, Asad U.
author_sort Beg, Ayesha Z.
collection PubMed
description BACKGROUND: Emergence of carbapenem resistant Escherichia coli pathovars and their environmental dissemination are alarming problems. E. coli isolated from sewage water of hospital setting conferred a high resistance towards β-lactams, particularly towards carbapenem. This prompted us to perform whole genome sequence analysis to investigate the antimicrobial determinants, pathogenicity status and mobile genetic elements associated with resistance genes. RESULTS: To the best of our knowledge this is the first report of ST 315 carrying NDM-4 from India. The genome analysis has revealed the unknown characteristics associated with this sequence type (ST 315) like resistance and virulence factors. Based on virulence markers, its pathotype was identified as ExPEC. Furthermore, a mobile plasmid with multiple β-lactamases genes and clinically relevant resistance markers was detected. Phylogenetic analysis of Inc F plasmids sequences carrying ESBLs and NDM variants, revealed un-relatedness in these plasmids due to their varying size and backbone sequences. CONCLUSIONS: Presence of carbapenem resistant E. coli ST 315 with high level antibiotic resistance, near hospital environment is an alarming situation in context to its spread. WGS based analyses have provided details on virulence and resistance status which could overcome the lack of information available on ST 315, globally. This could further help in its quick detection and control in clinical settings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0247-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-59684842018-05-30 Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals Beg, Ayesha Z. Khan, Asad U. Gut Pathog Genome Report BACKGROUND: Emergence of carbapenem resistant Escherichia coli pathovars and their environmental dissemination are alarming problems. E. coli isolated from sewage water of hospital setting conferred a high resistance towards β-lactams, particularly towards carbapenem. This prompted us to perform whole genome sequence analysis to investigate the antimicrobial determinants, pathogenicity status and mobile genetic elements associated with resistance genes. RESULTS: To the best of our knowledge this is the first report of ST 315 carrying NDM-4 from India. The genome analysis has revealed the unknown characteristics associated with this sequence type (ST 315) like resistance and virulence factors. Based on virulence markers, its pathotype was identified as ExPEC. Furthermore, a mobile plasmid with multiple β-lactamases genes and clinically relevant resistance markers was detected. Phylogenetic analysis of Inc F plasmids sequences carrying ESBLs and NDM variants, revealed un-relatedness in these plasmids due to their varying size and backbone sequences. CONCLUSIONS: Presence of carbapenem resistant E. coli ST 315 with high level antibiotic resistance, near hospital environment is an alarming situation in context to its spread. WGS based analyses have provided details on virulence and resistance status which could overcome the lack of information available on ST 315, globally. This could further help in its quick detection and control in clinical settings. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s13099-018-0247-8) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-24 /pmc/articles/PMC5968484/ /pubmed/29849769 http://dx.doi.org/10.1186/s13099-018-0247-8 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Genome Report
Beg, Ayesha Z.
Khan, Asad U.
Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals
title Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals
title_full Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals
title_fullStr Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals
title_full_unstemmed Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals
title_short Genome analyses of bla(NDM-4) carrying ST 315 Escherichia coli isolate from sewage water of one of the Indian hospitals
title_sort genome analyses of bla(ndm-4) carrying st 315 escherichia coli isolate from sewage water of one of the indian hospitals
topic Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968484/
https://www.ncbi.nlm.nih.gov/pubmed/29849769
http://dx.doi.org/10.1186/s13099-018-0247-8
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