Cargando…

Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging

BACKGROUND: Reverse genetic strategies, such as virus-induced gene silencing, are powerful techniques to study gene function. Currently, there are few tools to study the spatial dependence of the consequences of gene silencing at the cellular level. RESULTS: We report the use of multimodal Raman and...

Descripción completa

Detalles Bibliográficos
Autores principales:	Burkhow, Sadie J., Stephens, Nicole M., Mei, Yu, Dueñas, Maria Emilia, Freppon, Daniel J., Ding, Geng, Smith, Shea C., Lee, Young-Jin, Nikolau, Basil J., Whitham, Steven A., Smith, Emily A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2018
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968576/ https://www.ncbi.nlm.nih.gov/pubmed/29849743 http://dx.doi.org/10.1186/s13007-018-0306-7

Ejemplares similares

Combined use of cutinase and high-resolution mass-spectrometry to query the molecular architecture of cutin
por: Bhunia, Rupam Kumar, et al.
Publicado: (2018)

Complex Changes in Membrane Lipids Associated with the Modification of Autophagy in Arabidopsis
por: Mugume, Yosia, et al.
Publicado: (2022)

Correction: Mugume et al. Complex Changes in Membrane Lipids Associated with the Modification of Autophagy in Arabidopsis. Metabolites 2022, 12, 190
por: Mugume, Yosia, et al.
Publicado: (2022)

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots
por: Lentz, Ezequiel Matias, et al.
Publicado: (2018)

In vivo silencing of alpha-synuclein using naked siRNA
por: Lewis, Jada, et al.
Publicado: (2008)

An environmental transfer hub for multimodal atom probe tomography
por: Perea, Daniel E., et al.
Publicado: (2017)

A versatile multimodal chromatography strategy to rapidly purify protein nanostructures assembled in cell lysates
por: Winter, Daniel L., et al.
Publicado: (2023)

A rapid virus-induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease
por: Beyene, Getu, et al.
Publicado: (2017)

An effective virus-based gene silencing method for functional genomics studies in common bean
por: Díaz-Camino, Claudia, et al.
Publicado: (2011)

Improved apple latent spherical virus-induced gene silencing in multiple soybean genotypes through direct inoculation of agro-infiltrated Nicotiana benthamiana extract
por: Gedling, C. R., et al.
Publicado: (2018)

Development of a gene silencing DNA vector derived from a broad host range geminivirus
por: Golenberg, Edward M, et al.
Publicado: (2009)

Biological insights from multi-omics analysis strategies: Complex pleotropic effects associated with autophagy
por: Ding, Geng, et al.
Publicado: (2023)

3DCellAtlas Meristem: a tool for the global cellular annotation of shoot apical meristems
por: Montenegro-Johnson, Thomas, et al.
Publicado: (2019)

Dual patch voltage clamp study of low membrane resistance astrocytes in situ
por: Ma, Baofeng, et al.
Publicado: (2014)

Applying shot boundary detection for automated crystal growth analysis during in situ transmission electron microscope experiments
por: Moeglein, W. A., et al.
Publicado: (2017)

Protocol: a fast and simple in situ PCR method for localising gene expression in plant tissue
por: Athman, Asmini, et al.
Publicado: (2014)

In situ measurement of Scots pine needle PRI
por: Mõttus, Matti, et al.
Publicado: (2017)

Rapid, high efficiency virus-mediated mutant complementation and gene silencing in Antirrhinum
por: Tan, Ying, et al.
Publicado: (2020)

Comparison and extension of three methods for automated registration of multimodal plant images
por: Henke, Michael, et al.
Publicado: (2019)

A mixture copula Bayesian network model for multimodal genomic data
por: Zhang, Qingyang, et al.
Publicado: (2017)

Social disparities in heart disease risk and survivor bias among autoworkers: an examination based on survival models and g-estimation
por: Costello, Sadie, et al.
Publicado: (2015)

A cassava common mosaic virus vector for virus-induced gene silencing in cassava
por: Tuo, Decai, et al.
Publicado: (2021)

Foxtail mosaic virus-induced gene silencing (VIGS) in switchgrass (Panicum virgatum L.)
por: Tiedge, Kira, et al.
Publicado: (2022)

A CRISPR interference strategy for gene expression silencing in multiple myeloma cell lines
por: Escrivá-Fernández, Josep, et al.
Publicado: (2023)

RNAi-mediated gene silencing in tick synganglia: A proof of concept study
por: Karim, Shahid, et al.
Publicado: (2008)

Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs
por: Janssens, Derek H., et al.
Publicado: (2018)

Adaptive Multiview Nonnegative Matrix Factorization Algorithm for Integration of Multimodal Biomedical Data
por: Ray, Bisakha, et al.
Publicado: (2017)

Comparison of Vibratome and Compresstome sectioning of fresh primate lymphoid and genital tissues for in situ MHC-tetramer and immunofluorescence staining
por: Abdelaal, Hadia M, et al.
Publicado: (2015)

One-fits-all pretreatment protocol facilitating Fluorescence In Situ Hybridization on formalin-fixed paraffin-embedded, fresh frozen and cytological slides
por: Richardson, Shivanand O., et al.
Publicado: (2019)

In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections
por: Ellison, Mitchell A., et al.
Publicado: (2016)

An integrative Raman microscopy-based workflow for rapid in situ analysis of microalgal lipid bodies
por: Sharma, Sudhir Kumar, et al.
Publicado: (2015)

Direct DNA and PNA probe binding to telomeric regions without classical in situ hybridization
por: Genet, Matthew D, et al.
Publicado: (2013)

Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging
por: Stephan, Dirk, et al.
Publicado: (2011)

Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
por: Grønlund, Mette, et al.
Publicado: (2010)

Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system
por: Tuttle, John Richard, et al.
Publicado: (2012)

A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reesei
por: Wang, Lei, et al.
Publicado: (2018)

Hypothesis testing for evaluating a multimodal pattern recognition framework applied to speaker detection
por: Besson, Patricia, et al.
Publicado: (2008)

An in situ approach to characterizing photosynthetic gas exchange of rice panicle
por: Chang, Tian-Gen, et al.
Publicado: (2020)

Functional genomics tool: Gene silencing in Ixodes scapularis eggs and nymphs by electroporated dsRNA
por: Karim, Shahid, et al.
Publicado: (2010)

RNA interference-mediated gene silencing in murine T cells: in vitro and in vivo validation of proinflammatory target genes
por: Gust, Tatjana C, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_gfr1t55e2m6nc7cu2cjhn32naf