Cargando…
Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study
BACKGROUND: Innovating newer methods to diagnose a multifactorial disease such as periodontitis is always challenging for a clinician. Gingival crevicular fluid (GCF) which is closely associated with the periodontal tissue environment has been used a viable alternative to saliva for the diagnosis of...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968698/ https://www.ncbi.nlm.nih.gov/pubmed/29875576 http://dx.doi.org/10.4103/ccd.ccd_51_18 |
_version_ | 1783325823252561920 |
---|---|
author | Raj, Subash Chandra Panda, Subhashree Manaswini Dash, Muktikesh Patnaik, Kaushik Mohanty, Devapratim Katti, Neelima Mahapatra, Annuroopa Mishra, Debasish Praharaj, Kamdev |
author_facet | Raj, Subash Chandra Panda, Subhashree Manaswini Dash, Muktikesh Patnaik, Kaushik Mohanty, Devapratim Katti, Neelima Mahapatra, Annuroopa Mishra, Debasish Praharaj, Kamdev |
author_sort | Raj, Subash Chandra |
collection | PubMed |
description | BACKGROUND: Innovating newer methods to diagnose a multifactorial disease such as periodontitis is always challenging for a clinician. Gingival crevicular fluid (GCF) which is closely associated with the periodontal tissue environment has been used a viable alternative to saliva for the diagnosis of periodontitis. AIM: The aim of the present study was to estimate and compare the interleukin-35 (IL-35) levels in GCF and serum among healthy, gingivitis, and chronic periodontitis (CP) individuals as well as to evaluate the effect of nonsurgical periodontal treatment (NSPT) on IL-35 level among patients with CP. SETTINGS AND DESIGN: The study was conducted at the Department of Periodontics, Srirama Chandra Bhanja Dental College and Hospital, Cuttack, Odisha, India. It is a comparative study. MATERIALS AND METHODS: A total of 60 participants were divided into healthy (Group I; n = 20), gingivitis (Group II; n = 20), and CP (Group IIIA; n = 20). GCF samples collected from each individual at baseline and 6 weeks after NSPT for Group III individuals (Group IIIB; n = 20) were quantified for IL-35 levels using enzyme-linked immunosorbent assay. STATISTICAL ANALYSIS: All analyses were performed using Shapiro–Wilk test, analysis of variance, Tukey's honestly significant difference post hoc test, and multiple regression analysis. RESULTS: The mean IL-35 concentration in GCF was significantly high (P < 0.05) for Group IIIA (70.26 ± 4.0 pg/ml), as compared to Group I (54.81 ± 22.3 pg/ml) and Group IIIB (55.72 ± 10.2 pg/ml). CONCLUSION: In the present study, GCF and serum IL-35 concentration among CP individuals was highest among all the groups. Individuals receiving NSPT showed a significant reduction in IL-35 levels as compared to CP individuals. |
format | Online Article Text |
id | pubmed-5968698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-59686982018-06-06 Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study Raj, Subash Chandra Panda, Subhashree Manaswini Dash, Muktikesh Patnaik, Kaushik Mohanty, Devapratim Katti, Neelima Mahapatra, Annuroopa Mishra, Debasish Praharaj, Kamdev Contemp Clin Dent Original Article BACKGROUND: Innovating newer methods to diagnose a multifactorial disease such as periodontitis is always challenging for a clinician. Gingival crevicular fluid (GCF) which is closely associated with the periodontal tissue environment has been used a viable alternative to saliva for the diagnosis of periodontitis. AIM: The aim of the present study was to estimate and compare the interleukin-35 (IL-35) levels in GCF and serum among healthy, gingivitis, and chronic periodontitis (CP) individuals as well as to evaluate the effect of nonsurgical periodontal treatment (NSPT) on IL-35 level among patients with CP. SETTINGS AND DESIGN: The study was conducted at the Department of Periodontics, Srirama Chandra Bhanja Dental College and Hospital, Cuttack, Odisha, India. It is a comparative study. MATERIALS AND METHODS: A total of 60 participants were divided into healthy (Group I; n = 20), gingivitis (Group II; n = 20), and CP (Group IIIA; n = 20). GCF samples collected from each individual at baseline and 6 weeks after NSPT for Group III individuals (Group IIIB; n = 20) were quantified for IL-35 levels using enzyme-linked immunosorbent assay. STATISTICAL ANALYSIS: All analyses were performed using Shapiro–Wilk test, analysis of variance, Tukey's honestly significant difference post hoc test, and multiple regression analysis. RESULTS: The mean IL-35 concentration in GCF was significantly high (P < 0.05) for Group IIIA (70.26 ± 4.0 pg/ml), as compared to Group I (54.81 ± 22.3 pg/ml) and Group IIIB (55.72 ± 10.2 pg/ml). CONCLUSION: In the present study, GCF and serum IL-35 concentration among CP individuals was highest among all the groups. Individuals receiving NSPT showed a significant reduction in IL-35 levels as compared to CP individuals. Medknow Publications & Media Pvt Ltd 2018 /pmc/articles/PMC5968698/ /pubmed/29875576 http://dx.doi.org/10.4103/ccd.ccd_51_18 Text en Copyright: © 2018 Contemporary Clinical Dentistry http://creativecommons.org/licenses/by-nc-sa/4.0 This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Raj, Subash Chandra Panda, Subhashree Manaswini Dash, Muktikesh Patnaik, Kaushik Mohanty, Devapratim Katti, Neelima Mahapatra, Annuroopa Mishra, Debasish Praharaj, Kamdev Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study |
title | Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study |
title_full | Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study |
title_fullStr | Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study |
title_full_unstemmed | Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study |
title_short | Association of Human Interleukin-35 Level in Gingival Crevicular Fluid and Serum in Periodontal Health, Disease, and after Nonsurgical Therapy: A Comparative Study |
title_sort | association of human interleukin-35 level in gingival crevicular fluid and serum in periodontal health, disease, and after nonsurgical therapy: a comparative study |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5968698/ https://www.ncbi.nlm.nih.gov/pubmed/29875576 http://dx.doi.org/10.4103/ccd.ccd_51_18 |
work_keys_str_mv | AT rajsubashchandra associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT pandasubhashreemanaswini associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT dashmuktikesh associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT patnaikkaushik associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT mohantydevapratim associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT kattineelima associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT mahapatraannuroopa associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT mishradebasish associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy AT praharajkamdev associationofhumaninterleukin35levelingingivalcrevicularfluidandseruminperiodontalhealthdiseaseandafternonsurgicaltherapyacomparativestudy |