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Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection

Thioester‐containing proteins (TEPs) are conserved proteins with a role in innate immune immunity. In the current study, we characterized the TEP family in the genome of six tsetse fly species (Glossina spp.). Tsetse flies are the biological vectors of several African trypanosomes, which cause sleep...

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Autores principales: Matetovici, I., Van Den Abbeele, J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969219/
https://www.ncbi.nlm.nih.gov/pubmed/29528164
http://dx.doi.org/10.1111/imb.12382
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author Matetovici, I.
Van Den Abbeele, J.
author_facet Matetovici, I.
Van Den Abbeele, J.
author_sort Matetovici, I.
collection PubMed
description Thioester‐containing proteins (TEPs) are conserved proteins with a role in innate immune immunity. In the current study, we characterized the TEP family in the genome of six tsetse fly species (Glossina spp.). Tsetse flies are the biological vectors of several African trypanosomes, which cause sleeping sickness in humans or nagana in livestock. The analysis of the tsetse TEP sequences revealed information about their structure, evolutionary relationships and expression profiles under both normal and trypanosome infection conditions. Phylogenetic analysis of the family showed that tsetse flies harbour a genomic expansion of specific TEPs that are not found in other dipterans. We found a general expression of all TEP genes in the alimentary tract, mouthparts and salivary glands. Glossina morsitans and Glossina palpalis TEP genes display a tissue‐specific expression pattern with some that are markedly up‐regulated when the fly is infected with the trypanosome parasite. A different TEP response was observed to infection with Trypanosoma brucei compared to Trypanosoma congolense, indicating that the tsetse TEP response is trypanosome‐specific. These findings are suggestive for the involvement of the TEP family in tsetse innate immunity, with a possible role in the control of the trypanosome parasite.
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spelling pubmed-59692192018-05-30 Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection Matetovici, I. Van Den Abbeele, J. Insect Mol Biol Original Articles Thioester‐containing proteins (TEPs) are conserved proteins with a role in innate immune immunity. In the current study, we characterized the TEP family in the genome of six tsetse fly species (Glossina spp.). Tsetse flies are the biological vectors of several African trypanosomes, which cause sleeping sickness in humans or nagana in livestock. The analysis of the tsetse TEP sequences revealed information about their structure, evolutionary relationships and expression profiles under both normal and trypanosome infection conditions. Phylogenetic analysis of the family showed that tsetse flies harbour a genomic expansion of specific TEPs that are not found in other dipterans. We found a general expression of all TEP genes in the alimentary tract, mouthparts and salivary glands. Glossina morsitans and Glossina palpalis TEP genes display a tissue‐specific expression pattern with some that are markedly up‐regulated when the fly is infected with the trypanosome parasite. A different TEP response was observed to infection with Trypanosoma brucei compared to Trypanosoma congolense, indicating that the tsetse TEP response is trypanosome‐specific. These findings are suggestive for the involvement of the TEP family in tsetse innate immunity, with a possible role in the control of the trypanosome parasite. John Wiley and Sons Inc. 2018-03-12 2018-06 /pmc/articles/PMC5969219/ /pubmed/29528164 http://dx.doi.org/10.1111/imb.12382 Text en © 2018 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Matetovici, I.
Van Den Abbeele, J.
Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection
title Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection
title_full Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection
title_fullStr Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection
title_full_unstemmed Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection
title_short Thioester‐containing proteins in the tsetse fly (Glossina) and their response to trypanosome infection
title_sort thioester‐containing proteins in the tsetse fly (glossina) and their response to trypanosome infection
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969219/
https://www.ncbi.nlm.nih.gov/pubmed/29528164
http://dx.doi.org/10.1111/imb.12382
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