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A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron
We have generated a site‐directed mutant of the manganese superoxide dismutase SOD‐3 of C.elegans (MnSOD‐3) which modifies the metal specificity of the enzyme. While wild‐type MnSOD‐3 functions with manganese in the active site (3600 U mg(−1) of protein) it has little or no activity when iron is inc...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969255/ https://www.ncbi.nlm.nih.gov/pubmed/29178484 http://dx.doi.org/10.1002/chem.201704655 |
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| author | Hunter, Thérèse Bonetta, Rosalin Sacco, Anthony Vella, Marita Sultana, Paul‐Michael Trinh, Chi H. Fadia, Hava B. R. Borowski, Tomasz Garcia‐Fandiño, Rebeca Stockner, Thomas Hunter, Gary J. |
| author_facet | Hunter, Thérèse Bonetta, Rosalin Sacco, Anthony Vella, Marita Sultana, Paul‐Michael Trinh, Chi H. Fadia, Hava B. R. Borowski, Tomasz Garcia‐Fandiño, Rebeca Stockner, Thomas Hunter, Gary J. |
| author_sort | Hunter, Thérèse |
| collection | PubMed |
| description | We have generated a site‐directed mutant of the manganese superoxide dismutase SOD‐3 of C.elegans (MnSOD‐3) which modifies the metal specificity of the enzyme. While wild‐type MnSOD‐3 functions with manganese in the active site (3600 U mg(−1) of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50 % of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or iron. |
| format | Online Article Text |
| id | pubmed-5969255 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| publisher | John Wiley and Sons Inc. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-59692552018-05-30 A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron Hunter, Thérèse Bonetta, Rosalin Sacco, Anthony Vella, Marita Sultana, Paul‐Michael Trinh, Chi H. Fadia, Hava B. R. Borowski, Tomasz Garcia‐Fandiño, Rebeca Stockner, Thomas Hunter, Gary J. Chemistry Full Papers We have generated a site‐directed mutant of the manganese superoxide dismutase SOD‐3 of C.elegans (MnSOD‐3) which modifies the metal specificity of the enzyme. While wild‐type MnSOD‐3 functions with manganese in the active site (3600 U mg(−1) of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50 % of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or iron. John Wiley and Sons Inc. 2017-12-12 2018-04-06 /pmc/articles/PMC5969255/ /pubmed/29178484 http://dx.doi.org/10.1002/chem.201704655 Text en © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
| spellingShingle | Full Papers Hunter, Thérèse Bonetta, Rosalin Sacco, Anthony Vella, Marita Sultana, Paul‐Michael Trinh, Chi H. Fadia, Hava B. R. Borowski, Tomasz Garcia‐Fandiño, Rebeca Stockner, Thomas Hunter, Gary J. A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron |
| title | A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron |
| title_full | A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron |
| title_fullStr | A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron |
| title_full_unstemmed | A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron |
| title_short | A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron |
| title_sort | single mutation is sufficient to modify the metal selectivity and specificity of a eukaryotic manganese superoxide dismutase to encompass iron |
| topic | Full Papers |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5969255/ https://www.ncbi.nlm.nih.gov/pubmed/29178484 http://dx.doi.org/10.1002/chem.201704655 |
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