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Identification and characterization of microRNAs in the pituitary of pubescent goats

BACKGROUND: Puberty is the period during a female mammal’s life when it enters estrus and ovulates for the first time; this indicates that a mammal is capable of reproduction. The onset of puberty is a complex and tightly coordinated biological event; it has been reported that microRNAs (miRNAs) are...

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Autores principales: Ye, Jing, Yao, Zhiqiu, Si, Wenyu, Gao, Xiaoxiao, Yang, Chen, Liu, Ya, Ding, Jianping, Huang, Weiping, Fang, Fugui, Zhou, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5970454/
https://www.ncbi.nlm.nih.gov/pubmed/29801455
http://dx.doi.org/10.1186/s12958-018-0370-x
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author Ye, Jing
Yao, Zhiqiu
Si, Wenyu
Gao, Xiaoxiao
Yang, Chen
Liu, Ya
Ding, Jianping
Huang, Weiping
Fang, Fugui
Zhou, Jie
author_facet Ye, Jing
Yao, Zhiqiu
Si, Wenyu
Gao, Xiaoxiao
Yang, Chen
Liu, Ya
Ding, Jianping
Huang, Weiping
Fang, Fugui
Zhou, Jie
author_sort Ye, Jing
collection PubMed
description BACKGROUND: Puberty is the period during a female mammal’s life when it enters estrus and ovulates for the first time; this indicates that a mammal is capable of reproduction. The onset of puberty is a complex and tightly coordinated biological event; it has been reported that microRNAs (miRNAs) are involved in regulating the initiation of puberty. METHODS: We performed miRNA sequencing on pituitary tissue from prepubescent and pubescent goats to investigate differences in miRNA expression during the onset of puberty in female goats. The target genes of these miRNAs were evaluated by GO enrichment and KEGG pathway analysis to identify critical pathways regulated by these miRNAs during puberty in goats. Finally, we selected four known miRNA and one novel miRNAs to evaluate expression patterns in two samples via qRT-PCR to validate the RNA-seq data. RESULTS: In this study, 476 miRNAs were detected in goat pituitary tissue; 13 of these were specifically expressed in the pituitary of prepubescent goats, and 17 were unique to the pituitary of pubescent goats. Additionally, 73 novel miRNAs were predicted in these two libraries. 20 differentially expressed miRNAs were identified in this study. KEGG pathway enrichment analysis revealed that the differentially expressed miRNA target genes were enriched in pathways related to ovary development during puberty, including the GABAergic synapse, oxytocin signaling pathway, the cAMP signaling pathway, progesterone-mediated oocyte maturation. In this study, differential miRNA expression in the pituitary tissue of prepubescent and pubescent goats were identified and characterized. CONCLUSION: These results provide important information regarding the potential regulation of the onset of goat puberty by miRNAs, and contribute to the elucidation of miRNA regulated processes during maturation and reproduction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12958-018-0370-x) contains supplementary material, which is available to authorized users.
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spelling pubmed-59704542018-05-30 Identification and characterization of microRNAs in the pituitary of pubescent goats Ye, Jing Yao, Zhiqiu Si, Wenyu Gao, Xiaoxiao Yang, Chen Liu, Ya Ding, Jianping Huang, Weiping Fang, Fugui Zhou, Jie Reprod Biol Endocrinol Research BACKGROUND: Puberty is the period during a female mammal’s life when it enters estrus and ovulates for the first time; this indicates that a mammal is capable of reproduction. The onset of puberty is a complex and tightly coordinated biological event; it has been reported that microRNAs (miRNAs) are involved in regulating the initiation of puberty. METHODS: We performed miRNA sequencing on pituitary tissue from prepubescent and pubescent goats to investigate differences in miRNA expression during the onset of puberty in female goats. The target genes of these miRNAs were evaluated by GO enrichment and KEGG pathway analysis to identify critical pathways regulated by these miRNAs during puberty in goats. Finally, we selected four known miRNA and one novel miRNAs to evaluate expression patterns in two samples via qRT-PCR to validate the RNA-seq data. RESULTS: In this study, 476 miRNAs were detected in goat pituitary tissue; 13 of these were specifically expressed in the pituitary of prepubescent goats, and 17 were unique to the pituitary of pubescent goats. Additionally, 73 novel miRNAs were predicted in these two libraries. 20 differentially expressed miRNAs were identified in this study. KEGG pathway enrichment analysis revealed that the differentially expressed miRNA target genes were enriched in pathways related to ovary development during puberty, including the GABAergic synapse, oxytocin signaling pathway, the cAMP signaling pathway, progesterone-mediated oocyte maturation. In this study, differential miRNA expression in the pituitary tissue of prepubescent and pubescent goats were identified and characterized. CONCLUSION: These results provide important information regarding the potential regulation of the onset of goat puberty by miRNAs, and contribute to the elucidation of miRNA regulated processes during maturation and reproduction. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12958-018-0370-x) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-25 /pmc/articles/PMC5970454/ /pubmed/29801455 http://dx.doi.org/10.1186/s12958-018-0370-x Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Ye, Jing
Yao, Zhiqiu
Si, Wenyu
Gao, Xiaoxiao
Yang, Chen
Liu, Ya
Ding, Jianping
Huang, Weiping
Fang, Fugui
Zhou, Jie
Identification and characterization of microRNAs in the pituitary of pubescent goats
title Identification and characterization of microRNAs in the pituitary of pubescent goats
title_full Identification and characterization of microRNAs in the pituitary of pubescent goats
title_fullStr Identification and characterization of microRNAs in the pituitary of pubescent goats
title_full_unstemmed Identification and characterization of microRNAs in the pituitary of pubescent goats
title_short Identification and characterization of microRNAs in the pituitary of pubescent goats
title_sort identification and characterization of micrornas in the pituitary of pubescent goats
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5970454/
https://www.ncbi.nlm.nih.gov/pubmed/29801455
http://dx.doi.org/10.1186/s12958-018-0370-x
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