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Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model

BACKGROUND: Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation a...

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Autores principales: Hop, Huynh Tan, Arayan, Lauren Togonon, Reyes, Alisha Wehdnesday Bernardo, Huy, Tran Xuan Ngoc, Min, Won Gi, Lee, Hu Jang, Rhee, Man Hee, Chang, Hong Hee, Kim, Suk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5970535/
https://www.ncbi.nlm.nih.gov/pubmed/29801438
http://dx.doi.org/10.1186/s12866-018-1185-9
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author Hop, Huynh Tan
Arayan, Lauren Togonon
Reyes, Alisha Wehdnesday Bernardo
Huy, Tran Xuan Ngoc
Min, Won Gi
Lee, Hu Jang
Rhee, Man Hee
Chang, Hong Hee
Kim, Suk
author_facet Hop, Huynh Tan
Arayan, Lauren Togonon
Reyes, Alisha Wehdnesday Bernardo
Huy, Tran Xuan Ngoc
Min, Won Gi
Lee, Hu Jang
Rhee, Man Hee
Chang, Hong Hee
Kim, Suk
author_sort Hop, Huynh Tan
collection PubMed
description BACKGROUND: Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. RESULTS: RAW264.7 cells were incubated at 43 °C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1β and IFN-γ in the sera of the mice. Gene expression of NF-κB and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-κB and the expression of the heat shock proteins HSP70 and HSP90 in murine macrophages. Furthermore, heat exposure induced the increase of pro-inflammatory cytokines, ROS accumulation and apoptosis but did not affect the production of nitric oxide (NO) in macrophages. CONCLUSION: This study demonstrated the induction of innate immune responses by heat stress that significantly reduced the intracellular survival of B. abortus in vitro and in vivo. Transcriptional factor NF-κB, which is a master regulator, could be termed a key activator of heat-induced immunity against Brucella. The increase in the expression and activation of NF-κB in splenic cells and macrophages was followed by enhanced antimicrobial effectors, including cytokines, ROS and NO that may contribute to the reduction of bacterial survival.
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spelling pubmed-59705352018-05-30 Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model Hop, Huynh Tan Arayan, Lauren Togonon Reyes, Alisha Wehdnesday Bernardo Huy, Tran Xuan Ngoc Min, Won Gi Lee, Hu Jang Rhee, Man Hee Chang, Hong Hee Kim, Suk BMC Microbiol Research Article BACKGROUND: Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. RESULTS: RAW264.7 cells were incubated at 43 °C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1β and IFN-γ in the sera of the mice. Gene expression of NF-κB and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-κB and the expression of the heat shock proteins HSP70 and HSP90 in murine macrophages. Furthermore, heat exposure induced the increase of pro-inflammatory cytokines, ROS accumulation and apoptosis but did not affect the production of nitric oxide (NO) in macrophages. CONCLUSION: This study demonstrated the induction of innate immune responses by heat stress that significantly reduced the intracellular survival of B. abortus in vitro and in vivo. Transcriptional factor NF-κB, which is a master regulator, could be termed a key activator of heat-induced immunity against Brucella. The increase in the expression and activation of NF-κB in splenic cells and macrophages was followed by enhanced antimicrobial effectors, including cytokines, ROS and NO that may contribute to the reduction of bacterial survival. BioMed Central 2018-05-25 /pmc/articles/PMC5970535/ /pubmed/29801438 http://dx.doi.org/10.1186/s12866-018-1185-9 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Hop, Huynh Tan
Arayan, Lauren Togonon
Reyes, Alisha Wehdnesday Bernardo
Huy, Tran Xuan Ngoc
Min, Won Gi
Lee, Hu Jang
Rhee, Man Hee
Chang, Hong Hee
Kim, Suk
Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
title Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
title_full Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
title_fullStr Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
title_full_unstemmed Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
title_short Heat-stress-modulated induction of NF-κB leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
title_sort heat-stress-modulated induction of nf-κb leads to brucellacidal pro-inflammatory defense against brucella abortus infection in murine macrophages and in a mouse model
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5970535/
https://www.ncbi.nlm.nih.gov/pubmed/29801438
http://dx.doi.org/10.1186/s12866-018-1185-9
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