The Simultaneous Voltammetric Determination of Aflatoxins В(1) and М(1) on a Glassy-Carbon Electrode

For the first time, the possibility of using stripping voltammetry for the simultaneous determination of aflatoxins В(1) and М(1) on a glassy-carbon electrode has been shown. The influence of various factors (Е(э), τ(э), w, and the nature of the background electrolyte) on the potential and magnitude of the oxidation current of mycotoxins has been estimated. Working conditions for voltammetric determination and reproducibility of analytical signals for two mycotoxins have been selected. The mutual influence of aflatoxins В(1) and М(1) on the value of analytical signals in their simultaneous presence has been studied. It has been found that, in the range of their detectable contents, the presence of aflatoxin В(1) reduces the analytical signal of aflatoxin М(1) by 45–50%, but the linearity of the calibration dependence is preserved. The content of aflatoxin М(1) in determination of aflatoxin B(1) does not exert a significant effect in the range of 10–15%. Based on the results obtained, a procedure has been proposed for determining the content of aflatoxins В(1) and М(1) in their joint presence in milk by voltammetry in the concentration ranges 2 × 10(−3) ÷ 2 × 10(−1) mg/dm(3) and 2 × 10(−4) ÷ 2 × 10(−2) mg/dm(3), respectively (Sr not more than 18%).