A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants

BACKGROUND: The CRISPR/Cas9 system is being used for genome editing purposes by many research groups in multiple plant species. Traditional sequencing methods to identify homozygous mutants are time-consuming, laborious and expensive. RESULTS: We have developed a method to screen CRISPR/Cas9-induced...

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Detalles Bibliográficos
Autores principales: Guo, Jinggong, Li, Kun, Jin, Lifeng, Xu, Rui, Miao, Kaiting, Yang, Fengbo, Qi, Chaoya, Zhang, Lin, Botella, Jose R., Wang, Ran, Miao, Yuchen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972395/
https://www.ncbi.nlm.nih.gov/pubmed/29872452
http://dx.doi.org/10.1186/s13007-018-0305-8

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