CHROMIXS: automatic and interactive analysis of chromatography-coupled small-angle X-ray scattering data

SUMMARY: Size-exclusion chromatography (SEC) coupled to small-angle X-ray scattering (SAXS), also known as inline SEC-SAXS, is being increasingly used for the structural analysis of biological macromolecules, complexes and mixtures in solution. A single SEC-SAXS run generates thousands of individual SAXS profiles from the eluting solute and their analysis requires a correct identification of buffer and sample regions, a rather laborous task. We present CHROMIXS (as in CHROMatography Inline X-ray Scattering), a program for rapid reduction and analysis, both automatically and interactively, of SEC-SAXS data. AVAILABILITY AND IMPLEMENTATION: CHROMIXS is freely available to academic users as part of the ATSAS software suite (www.embl-hamburg.de/biosaxs/download.html).