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GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis

Cuticular wax is a major component of the surface cuticle of plants, which performs crucial functions in optimizing plant growth. Histone acetylation regulates gene expression in diverse biological processes, but its role in cuticular wax synthesis is not well understood. In this study, we observed...

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Autores principales: Wang, Tianya, Xing, Jiewen, Liu, Xinye, Yao, Yingyin, Hu, Zhaorong, Peng, Huiru, Xin, Mingming, Zhou, Dao-Xiu, Zhang, Yirong, Ni, Zhongfu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972625/
https://www.ncbi.nlm.nih.gov/pubmed/29506042
http://dx.doi.org/10.1093/jxb/ery077
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author Wang, Tianya
Xing, Jiewen
Liu, Xinye
Yao, Yingyin
Hu, Zhaorong
Peng, Huiru
Xin, Mingming
Zhou, Dao-Xiu
Zhang, Yirong
Ni, Zhongfu
author_facet Wang, Tianya
Xing, Jiewen
Liu, Xinye
Yao, Yingyin
Hu, Zhaorong
Peng, Huiru
Xin, Mingming
Zhou, Dao-Xiu
Zhang, Yirong
Ni, Zhongfu
author_sort Wang, Tianya
collection PubMed
description Cuticular wax is a major component of the surface cuticle of plants, which performs crucial functions in optimizing plant growth. Histone acetylation regulates gene expression in diverse biological processes, but its role in cuticular wax synthesis is not well understood. In this study, we observed that mutations of the Arabidopsis thaliana histone acetyltransferase GENERAL CONTROL NON-REPRESSED PROTEIN5 (GCN5) impaired the accumulation of stem cuticular wax. Three target genes of GCN5, ECERIFERUM3 (CER3), CER26, and CER1-LIKE1 (CER1-L1), were identified by RNA-seq and ChIP assays. H3K9/14 acetylation levels at the promoter regions of CER3, CER26, and CER1-L1 were consistently and significantly decreased in the gcn5-2 mutant as compared to the wild-type. Notably, overexpression of CER3 in the gcn5-2 mutant rescued the defect in stem cuticular wax biosynthesis. Collectively, these data demonstrate that GCN5 is involved in stem cuticular wax accumulation by modulating CER3 expression via H3K9/14 acetylation, which underlines the important role of histone acetylation in cuticular wax biosynthesis.
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spelling pubmed-59726252018-06-04 GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis Wang, Tianya Xing, Jiewen Liu, Xinye Yao, Yingyin Hu, Zhaorong Peng, Huiru Xin, Mingming Zhou, Dao-Xiu Zhang, Yirong Ni, Zhongfu J Exp Bot Research Papers Cuticular wax is a major component of the surface cuticle of plants, which performs crucial functions in optimizing plant growth. Histone acetylation regulates gene expression in diverse biological processes, but its role in cuticular wax synthesis is not well understood. In this study, we observed that mutations of the Arabidopsis thaliana histone acetyltransferase GENERAL CONTROL NON-REPRESSED PROTEIN5 (GCN5) impaired the accumulation of stem cuticular wax. Three target genes of GCN5, ECERIFERUM3 (CER3), CER26, and CER1-LIKE1 (CER1-L1), were identified by RNA-seq and ChIP assays. H3K9/14 acetylation levels at the promoter regions of CER3, CER26, and CER1-L1 were consistently and significantly decreased in the gcn5-2 mutant as compared to the wild-type. Notably, overexpression of CER3 in the gcn5-2 mutant rescued the defect in stem cuticular wax biosynthesis. Collectively, these data demonstrate that GCN5 is involved in stem cuticular wax accumulation by modulating CER3 expression via H3K9/14 acetylation, which underlines the important role of histone acetylation in cuticular wax biosynthesis. Oxford University Press 2018-05-25 2018-03-01 /pmc/articles/PMC5972625/ /pubmed/29506042 http://dx.doi.org/10.1093/jxb/ery077 Text en © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Wang, Tianya
Xing, Jiewen
Liu, Xinye
Yao, Yingyin
Hu, Zhaorong
Peng, Huiru
Xin, Mingming
Zhou, Dao-Xiu
Zhang, Yirong
Ni, Zhongfu
GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis
title GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis
title_full GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis
title_fullStr GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis
title_full_unstemmed GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis
title_short GCN5 contributes to stem cuticular wax biosynthesis by histone acetylation of CER3 in Arabidopsis
title_sort gcn5 contributes to stem cuticular wax biosynthesis by histone acetylation of cer3 in arabidopsis
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972625/
https://www.ncbi.nlm.nih.gov/pubmed/29506042
http://dx.doi.org/10.1093/jxb/ery077
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