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HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology
Understanding the neural mechanisms underlying human cognition and determining the causal factors for the development of brain pathologies are among the greatest challenges for society. Electrophysiological recordings offer remarkable observations of brain activity as they provide highly precise rep...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972628/ https://www.ncbi.nlm.nih.gov/pubmed/29872379 http://dx.doi.org/10.3389/fncir.2018.00041 |
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author | Delcasso, Sebastien Denagamage, Sachira Britton, Zelie Graybiel, Ann M. |
author_facet | Delcasso, Sebastien Denagamage, Sachira Britton, Zelie Graybiel, Ann M. |
author_sort | Delcasso, Sebastien |
collection | PubMed |
description | Understanding the neural mechanisms underlying human cognition and determining the causal factors for the development of brain pathologies are among the greatest challenges for society. Electrophysiological recordings offer remarkable observations of brain activity as they provide highly precise representations of information coding in both temporal and spatial domains. With the development of genetic tools over the last decades, mice have been a key model organism in neuroscience. However, conducting chronic in vivo electrophysiology in awake, behaving mice remains technically challenging, and this difficulty prevents many research teams from acquiring critical recordings in their mouse models. Behavioral training, implant fabrication, brain surgery, data acquisition and data analysis are all required steps that must be mastered in order to perform cutting-edge experiments in systems neuroscience. Here, we present a new method that simplifies the construction of a drivable and multi-task electrophysiological recording implant without loss of flexibility and recording power. The hybrid-drive combining optogenetics, pharmacology and electrophysiology (HOPE) can support up to 16 tetrodes, attached to a single drive mechanism, organized in two bundles of eight tetrodes, allowing recordings in two different mouse brain regions simultaneously with two optical fibers for optogenetic manipulation or two injection cannulas for drug-delivery experiments. Because it can be printed with a latest-generation desktop 3D printer, the production cost is low compared to classical electrophysiology implants, and it can be built within a few hours. The HOPE implant is also reconfigurable to specific needs as it has been created in a computer-aided design (CAD) software and all the files used for its construction are open-source. |
format | Online Article Text |
id | pubmed-5972628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-59726282018-06-05 HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology Delcasso, Sebastien Denagamage, Sachira Britton, Zelie Graybiel, Ann M. Front Neural Circuits Neuroscience Understanding the neural mechanisms underlying human cognition and determining the causal factors for the development of brain pathologies are among the greatest challenges for society. Electrophysiological recordings offer remarkable observations of brain activity as they provide highly precise representations of information coding in both temporal and spatial domains. With the development of genetic tools over the last decades, mice have been a key model organism in neuroscience. However, conducting chronic in vivo electrophysiology in awake, behaving mice remains technically challenging, and this difficulty prevents many research teams from acquiring critical recordings in their mouse models. Behavioral training, implant fabrication, brain surgery, data acquisition and data analysis are all required steps that must be mastered in order to perform cutting-edge experiments in systems neuroscience. Here, we present a new method that simplifies the construction of a drivable and multi-task electrophysiological recording implant without loss of flexibility and recording power. The hybrid-drive combining optogenetics, pharmacology and electrophysiology (HOPE) can support up to 16 tetrodes, attached to a single drive mechanism, organized in two bundles of eight tetrodes, allowing recordings in two different mouse brain regions simultaneously with two optical fibers for optogenetic manipulation or two injection cannulas for drug-delivery experiments. Because it can be printed with a latest-generation desktop 3D printer, the production cost is low compared to classical electrophysiology implants, and it can be built within a few hours. The HOPE implant is also reconfigurable to specific needs as it has been created in a computer-aided design (CAD) software and all the files used for its construction are open-source. Frontiers Media S.A. 2018-05-16 /pmc/articles/PMC5972628/ /pubmed/29872379 http://dx.doi.org/10.3389/fncir.2018.00041 Text en Copyright © 2018 Delcasso, Denagamage, Britton and Graybiel. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Delcasso, Sebastien Denagamage, Sachira Britton, Zelie Graybiel, Ann M. HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology |
title | HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology |
title_full | HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology |
title_fullStr | HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology |
title_full_unstemmed | HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology |
title_short | HOPE: Hybrid-Drive Combining Optogenetics, Pharmacology and Electrophysiology |
title_sort | hope: hybrid-drive combining optogenetics, pharmacology and electrophysiology |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972628/ https://www.ncbi.nlm.nih.gov/pubmed/29872379 http://dx.doi.org/10.3389/fncir.2018.00041 |
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