Cargando…

Specificity of peptidases secreted by filamentous fungi

Peptidases are enzymes that cleave peptide bonds, yielding proteins and peptides. Enzymes in this class also perform several other functions, regulating the activation or inactivation of target substrates via proteolysis. Owing to these functions, peptidases have been extensively used in industrial...

Descripción completa

Detalles Bibliográficos
Autores principales: Hamin Neto, Youssef Ali Abou, da Rosa Garzon, Nathália Gonsales, Pedezzi, Rafael, Cabral, Hamilton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972931/
https://www.ncbi.nlm.nih.gov/pubmed/28857638
http://dx.doi.org/10.1080/21655979.2017.1373531
_version_ 1783326496508608512
author Hamin Neto, Youssef Ali Abou
da Rosa Garzon, Nathália Gonsales
Pedezzi, Rafael
Cabral, Hamilton
author_facet Hamin Neto, Youssef Ali Abou
da Rosa Garzon, Nathália Gonsales
Pedezzi, Rafael
Cabral, Hamilton
author_sort Hamin Neto, Youssef Ali Abou
collection PubMed
description Peptidases are enzymes that cleave peptide bonds, yielding proteins and peptides. Enzymes in this class also perform several other functions, regulating the activation or inactivation of target substrates via proteolysis. Owing to these functions, peptidases have been extensively used in industrial and biotechnological applications. Given their potential functions, it is important to optimize the use of these enzymes, which requires determination of the specificity of each peptidase. The peptidase specificity must be taken into account in choosing a peptidase to catalyze the available protein source within the desired application. The specificity of a peptidase defines the profile of enzyme–substrate interactions, and for this the catalytic site and the arrangement of the amino acid residues involved in peptide bond cleavage need to be known. The catalytic sites of peptidases may be composed of several subsites that interact with amino acid residues for proteolysis. Filamentous fungi produce peptidases with varying specificity, and here we provide a review of those reported to date and their potential applications.
format Online
Article
Text
id pubmed-5972931
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-59729312018-09-21 Specificity of peptidases secreted by filamentous fungi Hamin Neto, Youssef Ali Abou da Rosa Garzon, Nathália Gonsales Pedezzi, Rafael Cabral, Hamilton Bioengineered Commentary Peptidases are enzymes that cleave peptide bonds, yielding proteins and peptides. Enzymes in this class also perform several other functions, regulating the activation or inactivation of target substrates via proteolysis. Owing to these functions, peptidases have been extensively used in industrial and biotechnological applications. Given their potential functions, it is important to optimize the use of these enzymes, which requires determination of the specificity of each peptidase. The peptidase specificity must be taken into account in choosing a peptidase to catalyze the available protein source within the desired application. The specificity of a peptidase defines the profile of enzyme–substrate interactions, and for this the catalytic site and the arrangement of the amino acid residues involved in peptide bond cleavage need to be known. The catalytic sites of peptidases may be composed of several subsites that interact with amino acid residues for proteolysis. Filamentous fungi produce peptidases with varying specificity, and here we provide a review of those reported to date and their potential applications. Taylor & Francis 2017-09-21 /pmc/articles/PMC5972931/ /pubmed/28857638 http://dx.doi.org/10.1080/21655979.2017.1373531 Text en © 2018 The Author(s). Published with license by Taylor & Francis http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Commentary
Hamin Neto, Youssef Ali Abou
da Rosa Garzon, Nathália Gonsales
Pedezzi, Rafael
Cabral, Hamilton
Specificity of peptidases secreted by filamentous fungi
title Specificity of peptidases secreted by filamentous fungi
title_full Specificity of peptidases secreted by filamentous fungi
title_fullStr Specificity of peptidases secreted by filamentous fungi
title_full_unstemmed Specificity of peptidases secreted by filamentous fungi
title_short Specificity of peptidases secreted by filamentous fungi
title_sort specificity of peptidases secreted by filamentous fungi
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972931/
https://www.ncbi.nlm.nih.gov/pubmed/28857638
http://dx.doi.org/10.1080/21655979.2017.1373531
work_keys_str_mv AT haminnetoyoussefaliabou specificityofpeptidasessecretedbyfilamentousfungi
AT darosagarzonnathaliagonsales specificityofpeptidasessecretedbyfilamentousfungi
AT pedezzirafael specificityofpeptidasessecretedbyfilamentousfungi
AT cabralhamilton specificityofpeptidasessecretedbyfilamentousfungi