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Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis

Saccharification potential of xylanase enzyme cloned from Bacillus licheniformis into E. coli BL21 (DE3) was evaluated against plant biomass for the production of bioethanol. The expression of cloned gene was studied and conditions were optimized for its large scale production. The parameters effect...

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Autores principales: Aftab, Muhammad N., Zafar, Asma, Iqbal, Irfana, Kaleem, Afshan, Zia, Khalid M., Awan, Ali R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972937/
https://www.ncbi.nlm.nih.gov/pubmed/28886289
http://dx.doi.org/10.1080/21655979.2017.1373918
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author Aftab, Muhammad N.
Zafar, Asma
Iqbal, Irfana
Kaleem, Afshan
Zia, Khalid M.
Awan, Ali R.
author_facet Aftab, Muhammad N.
Zafar, Asma
Iqbal, Irfana
Kaleem, Afshan
Zia, Khalid M.
Awan, Ali R.
author_sort Aftab, Muhammad N.
collection PubMed
description Saccharification potential of xylanase enzyme cloned from Bacillus licheniformis into E. coli BL21 (DE3) was evaluated against plant biomass for the production of bioethanol. The expression of cloned gene was studied and conditions were optimized for its large scale production. The parameters effecting enzyme production were examined in a fermenter. Recombinant xylanase has the ability to breakdown birchwood xylan to release xylose as well as the potential to treat plant biomass, such as wheat straw, rice straw, and sugarcane bagass. The saccharification ability of this enzyme was optimized by studying various parameters. The maximum saccharification percentage (84%) was achieved when 20 units of recombinant xylanase were used with 8% sugarcane bagass at 50°C and 120 rpm after 6 hours of incubation. The results indicated that the bioconversion of natural biomass by recombinant xylanase into simple sugars can be used for biofuel (bioethanol) production. This process can replace the use of fossil fuels, and the use of bioethanol can significantly reduce the emission of toxic gases. Future directions regarding pre-treatment of cellulosic and hemicellulosic biomass and other processes that can reduce the cost and enhance the yield of biofuels are briefly discussed.
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spelling pubmed-59729372018-09-28 Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis Aftab, Muhammad N. Zafar, Asma Iqbal, Irfana Kaleem, Afshan Zia, Khalid M. Awan, Ali R. Bioengineered Addendum Saccharification potential of xylanase enzyme cloned from Bacillus licheniformis into E. coli BL21 (DE3) was evaluated against plant biomass for the production of bioethanol. The expression of cloned gene was studied and conditions were optimized for its large scale production. The parameters effecting enzyme production were examined in a fermenter. Recombinant xylanase has the ability to breakdown birchwood xylan to release xylose as well as the potential to treat plant biomass, such as wheat straw, rice straw, and sugarcane bagass. The saccharification ability of this enzyme was optimized by studying various parameters. The maximum saccharification percentage (84%) was achieved when 20 units of recombinant xylanase were used with 8% sugarcane bagass at 50°C and 120 rpm after 6 hours of incubation. The results indicated that the bioconversion of natural biomass by recombinant xylanase into simple sugars can be used for biofuel (bioethanol) production. This process can replace the use of fossil fuels, and the use of bioethanol can significantly reduce the emission of toxic gases. Future directions regarding pre-treatment of cellulosic and hemicellulosic biomass and other processes that can reduce the cost and enhance the yield of biofuels are briefly discussed. Taylor & Francis 2017-09-28 /pmc/articles/PMC5972937/ /pubmed/28886289 http://dx.doi.org/10.1080/21655979.2017.1373918 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Addendum
Aftab, Muhammad N.
Zafar, Asma
Iqbal, Irfana
Kaleem, Afshan
Zia, Khalid M.
Awan, Ali R.
Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis
title Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis
title_full Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis
title_fullStr Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis
title_full_unstemmed Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis
title_short Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis
title_sort optimization of saccharification potential of recombinant xylanase from bacillus licheniformis
topic Addendum
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972937/
https://www.ncbi.nlm.nih.gov/pubmed/28886289
http://dx.doi.org/10.1080/21655979.2017.1373918
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