系统性鉴定长非编码RNA MALAT1调控的微小RNA

BACKGROUND AND OBJECTIVE: Long non-coding RNA (lncRNA) plays important regulatory roles in the development and invasion of various cancers. The aim of current study is to comprehensively identify microRNAs (miRNA) regulated by lncRNA MALAT1 via experimental and bioinformatics methods. METHODS: Antisense oligonucleotides (ASO) specifically targeting MALAT1 were designed and synthesized. After knockdown of MALAT1 by ASO in A549 cells, miRNA expression changes were profiled by TqaMan Low Density Array (TLDA). Gene set enrichment analysis (GSEA) was used to search enriched miRNAs among differentially expressed genes after knockdown of MALAT1. RESULTS: After efficient knockdown of MALAT1 by ASO, 153 miRNAs were differentially expressed, 131 up-regulated and 22 down-regulated. Among the 458 differentially expressed genes after MALAT1 silence, GSEA results revealed lots of enriched miRNAs. There were 28 overlapped miRNAs between TLDA and GSEA results, suggesting these 28 miRNAs are regulated by MALAT1. CONCLUSION: This study comprehensively identified MALAT1 regulated miRNAs, providing resources for further research.