Super-resolution binding activated localization microscopy through reversible change of DNA conformation

Methods of super-resolving light microscopy (SRM) have found an exponentially growing range of applications in cell biology, including nuclear structure analyses. Recent developments have proven that Single Molecule Localization Microscopy (SMLM), a type of SRM, is particularly useful for enhanced s...

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Autores principales: Szczurek, Aleksander, Birk, Udo, Knecht, Hans, Dobrucki, Jurek, Mai, Sabine, Cremer, Christoph
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2018
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973136/
https://www.ncbi.nlm.nih.gov/pubmed/29297245
http://dx.doi.org/10.1080/19491034.2017.1419846
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author Szczurek, Aleksander
Birk, Udo
Knecht, Hans
Dobrucki, Jurek
Mai, Sabine
Cremer, Christoph
author_facet Szczurek, Aleksander
Birk, Udo
Knecht, Hans
Dobrucki, Jurek
Mai, Sabine
Cremer, Christoph
author_sort Szczurek, Aleksander
collection PubMed
description Methods of super-resolving light microscopy (SRM) have found an exponentially growing range of applications in cell biology, including nuclear structure analyses. Recent developments have proven that Single Molecule Localization Microscopy (SMLM), a type of SRM, is particularly useful for enhanced spatial analysis of the cell nucleus due to its highest resolving capability combined with very specific fluorescent labeling. In this commentary we offer a brief review of the latest methodological development in the field of SMLM of chromatin designated DNA Structure Fluctuation Assisted Binding Activated Localization Microscopy (abbreviated as fBALM) as well as its potential future applications in biology and medicine.
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spelling pubmed-59731362018-05-31 Super-resolution binding activated localization microscopy through reversible change of DNA conformation Szczurek, Aleksander Birk, Udo Knecht, Hans Dobrucki, Jurek Mai, Sabine Cremer, Christoph Nucleus Extra View Methods of super-resolving light microscopy (SRM) have found an exponentially growing range of applications in cell biology, including nuclear structure analyses. Recent developments have proven that Single Molecule Localization Microscopy (SMLM), a type of SRM, is particularly useful for enhanced spatial analysis of the cell nucleus due to its highest resolving capability combined with very specific fluorescent labeling. In this commentary we offer a brief review of the latest methodological development in the field of SMLM of chromatin designated DNA Structure Fluctuation Assisted Binding Activated Localization Microscopy (abbreviated as fBALM) as well as its potential future applications in biology and medicine. Taylor & Francis 2018-03-01 /pmc/articles/PMC5973136/ /pubmed/29297245 http://dx.doi.org/10.1080/19491034.2017.1419846 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Extra View
Szczurek, Aleksander
Birk, Udo
Knecht, Hans
Dobrucki, Jurek
Mai, Sabine
Cremer, Christoph
Super-resolution binding activated localization microscopy through reversible change of DNA conformation
title Super-resolution binding activated localization microscopy through reversible change of DNA conformation
title_full Super-resolution binding activated localization microscopy through reversible change of DNA conformation
title_fullStr Super-resolution binding activated localization microscopy through reversible change of DNA conformation
title_full_unstemmed Super-resolution binding activated localization microscopy through reversible change of DNA conformation
title_short Super-resolution binding activated localization microscopy through reversible change of DNA conformation
title_sort super-resolution binding activated localization microscopy through reversible change of dna conformation
topic Extra View
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973136/
https://www.ncbi.nlm.nih.gov/pubmed/29297245
http://dx.doi.org/10.1080/19491034.2017.1419846
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