一种快速检测UGT1A1(*)28基因多态性的方法

BACKGROUND AND OBJECTIVE: Uridine-diphosphoglucuronosyl transferase 1A1 (UGT1A1), UGT1A1(*)28 polymorphism can reduce UGT1A1 enzymatic activity, which may lead to severe toxicities in patients who receive irinotecan. This study tries to build a fragment analysis method to detect UGT1A1(*)28 polymorp...

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Detalles Bibliográficos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 中国肺癌杂志编辑部 2017
Materias:
临床研究
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973388/
https://www.ncbi.nlm.nih.gov/pubmed/29277179
http://dx.doi.org/10.3779/j.issn.1009-3419.2017.12.04
Descripción
Sumario:BACKGROUND AND OBJECTIVE: Uridine-diphosphoglucuronosyl transferase 1A1 (UGT1A1), UGT1A1(*)28 polymorphism can reduce UGT1A1 enzymatic activity, which may lead to severe toxicities in patients who receive irinotecan. This study tries to build a fragment analysis method to detect UGT1A1(*)28 polymorphism. METHODS: A total of 286 blood specimens from the lung cancer patients who were hospitalized in Guangdong General Hospital between April 2014 to May 2015 were detected UGT1A1(*)28 polymorphism by fragment analysis method. RESULTS: Comparing with Sanger sequencing, precision and accuracy of the fragment analysis method were 100%. Of the 286 patients, 236 (82.5% harbored TA6/6 genotype, 48 (16.8%) TA 6/7 genotype and 2 (0.7%) TA7/7 genotype. CONCLUSION: Our data suggest hat the fragment analysis method is robust for detecting UGT1A1(*)28 polymorphism in clinical practice. It's simple, time-saving, and easy-to-carry.

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