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MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis
Mouse models of breast cancer with specific molecular subtypes (e.g., ER or HER2 positive) in an immunocompetent or an immunocompromised environment significantly contribute to our understanding of cancer biology, despite some limitations, and they give insight into targeted therapies. However, an i...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973560/ https://www.ncbi.nlm.nih.gov/pubmed/29813119 http://dx.doi.org/10.1371/journal.pone.0198143 |
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author | Ghosh, Arnab Sarkar, Sandipto Banerjee, Snigdha Behbod, Fariba Tawfik, Ossama McGregor, Douglas Graff, Stephanie Banerjee, Sushanta K. |
author_facet | Ghosh, Arnab Sarkar, Sandipto Banerjee, Snigdha Behbod, Fariba Tawfik, Ossama McGregor, Douglas Graff, Stephanie Banerjee, Sushanta K. |
author_sort | Ghosh, Arnab |
collection | PubMed |
description | Mouse models of breast cancer with specific molecular subtypes (e.g., ER or HER2 positive) in an immunocompetent or an immunocompromised environment significantly contribute to our understanding of cancer biology, despite some limitations, and they give insight into targeted therapies. However, an ideal triple-negative breast cancer (TNBC) mouse model is lacking. What has been missing in the TNBC mouse model is a sequential progression of the disease in an essential native microenvironment. This notion inspired us to develop a TNBC-model in syngeneic mice using a mammary intraductal (MIND) method. To achieve this goal, Mvt-1and 4T1 TNBC mouse cell lines were injected into the mammary ducts via nipples of FVB/N mice and BALB/c wild-type immunocompetent mice, respectively. We established that the TNBC-MIND model in syngeneic mice could epitomize all breast cancer progression stages and metastasis into the lungs via lymphatic or hematogenous dissemination within four weeks. Collectively, the syngeneic mouse-TNBC-MIND model may serve as a unique platform for further investigation of the underlying mechanisms of TNBC growth and therapies. |
format | Online Article Text |
id | pubmed-5973560 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-59735602018-06-08 MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis Ghosh, Arnab Sarkar, Sandipto Banerjee, Snigdha Behbod, Fariba Tawfik, Ossama McGregor, Douglas Graff, Stephanie Banerjee, Sushanta K. PLoS One Research Article Mouse models of breast cancer with specific molecular subtypes (e.g., ER or HER2 positive) in an immunocompetent or an immunocompromised environment significantly contribute to our understanding of cancer biology, despite some limitations, and they give insight into targeted therapies. However, an ideal triple-negative breast cancer (TNBC) mouse model is lacking. What has been missing in the TNBC mouse model is a sequential progression of the disease in an essential native microenvironment. This notion inspired us to develop a TNBC-model in syngeneic mice using a mammary intraductal (MIND) method. To achieve this goal, Mvt-1and 4T1 TNBC mouse cell lines were injected into the mammary ducts via nipples of FVB/N mice and BALB/c wild-type immunocompetent mice, respectively. We established that the TNBC-MIND model in syngeneic mice could epitomize all breast cancer progression stages and metastasis into the lungs via lymphatic or hematogenous dissemination within four weeks. Collectively, the syngeneic mouse-TNBC-MIND model may serve as a unique platform for further investigation of the underlying mechanisms of TNBC growth and therapies. Public Library of Science 2018-05-29 /pmc/articles/PMC5973560/ /pubmed/29813119 http://dx.doi.org/10.1371/journal.pone.0198143 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Ghosh, Arnab Sarkar, Sandipto Banerjee, Snigdha Behbod, Fariba Tawfik, Ossama McGregor, Douglas Graff, Stephanie Banerjee, Sushanta K. MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
title | MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
title_full | MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
title_fullStr | MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
title_full_unstemmed | MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
title_short | MIND model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
title_sort | mind model for triple-negative breast cancer in syngeneic mice for quick and sequential progression analysis of lung metastasis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973560/ https://www.ncbi.nlm.nih.gov/pubmed/29813119 http://dx.doi.org/10.1371/journal.pone.0198143 |
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