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Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity
AIM: The aim of this study was to investigate the impact of weight loss following gastric band surgery on multiple measures of peripheral blood neutrophil (PBN) function. MATERIAL AND METHODS: Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973997/ https://www.ncbi.nlm.nih.gov/pubmed/29238916 http://dx.doi.org/10.1007/s11695-017-3063-1 |
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author | Roberts, Helen M. Grant, Melissa M. Hubber, Naomi Super, Paul Singhal, Rishi Chapple, Iain L. C. |
author_facet | Roberts, Helen M. Grant, Melissa M. Hubber, Naomi Super, Paul Singhal, Rishi Chapple, Iain L. C. |
author_sort | Roberts, Helen M. |
collection | PubMed |
description | AIM: The aim of this study was to investigate the impact of weight loss following gastric band surgery on multiple measures of peripheral blood neutrophil (PBN) function. MATERIAL AND METHODS: Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention study, alongside non-obese, healthy gender- and age-matched controls. Eighteen pairs of patients and controls completed all stages of the study. PBNs were isolated by density centrifugation and a comprehensive analysis of PBN function was undertaken at various stages of the patients’ bariatric surgical care pathway. RESULTS: Obese patients exhibited exaggerated PBN activity in response to various stimuli, characterised by higher reactive oxygen species (ROS) generation (n = 18, p < 0.001) and release of pro-inflammatory cytokines (n = 10, p < 0.05) and lower PBN extracellular trap (NET) formation (n = 18, p < 0.01). PBN chemotactic accuracy was also impaired prior to surgery (n = 18, p < 0.01). Weight loss was associated with normalised NET production and lower ROS production and cytokine release relative to healthy controls. However, chemotactic accuracy remained impaired in patients. CONCLUSIONS: Weight loss following gastric band surgery was associated with a decrease in the pro-inflammatory activities of peripheral blood neutrophils (PBNs). A hyper-inflammatory PBN phenotype, involving excess ROS and cytokine release, reduced NET formation and chemotaxis, may lead to a reduced ability to eliminate infection, alongside inflammation-mediated tissue damage in obese individuals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11695-017-3063-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-5973997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-59739972018-06-08 Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity Roberts, Helen M. Grant, Melissa M. Hubber, Naomi Super, Paul Singhal, Rishi Chapple, Iain L. C. Obes Surg Original Contributions AIM: The aim of this study was to investigate the impact of weight loss following gastric band surgery on multiple measures of peripheral blood neutrophil (PBN) function. MATERIAL AND METHODS: Twenty-three obese patients undergoing gastric band surgery were recruited to a longitudinal intervention study, alongside non-obese, healthy gender- and age-matched controls. Eighteen pairs of patients and controls completed all stages of the study. PBNs were isolated by density centrifugation and a comprehensive analysis of PBN function was undertaken at various stages of the patients’ bariatric surgical care pathway. RESULTS: Obese patients exhibited exaggerated PBN activity in response to various stimuli, characterised by higher reactive oxygen species (ROS) generation (n = 18, p < 0.001) and release of pro-inflammatory cytokines (n = 10, p < 0.05) and lower PBN extracellular trap (NET) formation (n = 18, p < 0.01). PBN chemotactic accuracy was also impaired prior to surgery (n = 18, p < 0.01). Weight loss was associated with normalised NET production and lower ROS production and cytokine release relative to healthy controls. However, chemotactic accuracy remained impaired in patients. CONCLUSIONS: Weight loss following gastric band surgery was associated with a decrease in the pro-inflammatory activities of peripheral blood neutrophils (PBNs). A hyper-inflammatory PBN phenotype, involving excess ROS and cytokine release, reduced NET formation and chemotaxis, may lead to a reduced ability to eliminate infection, alongside inflammation-mediated tissue damage in obese individuals. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s11695-017-3063-1) contains supplementary material, which is available to authorized users. Springer US 2017-12-14 2018 /pmc/articles/PMC5973997/ /pubmed/29238916 http://dx.doi.org/10.1007/s11695-017-3063-1 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Contributions Roberts, Helen M. Grant, Melissa M. Hubber, Naomi Super, Paul Singhal, Rishi Chapple, Iain L. C. Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity |
title | Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity |
title_full | Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity |
title_fullStr | Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity |
title_full_unstemmed | Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity |
title_short | Impact of Bariatric Surgical Intervention on Peripheral Blood Neutrophil (PBN) Function in Obesity |
title_sort | impact of bariatric surgical intervention on peripheral blood neutrophil (pbn) function in obesity |
topic | Original Contributions |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973997/ https://www.ncbi.nlm.nih.gov/pubmed/29238916 http://dx.doi.org/10.1007/s11695-017-3063-1 |
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