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Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis

Lepidopteran insects use sex pheromones for sexual communication. Pheromone receptors expressed on peripheral olfactory receptor neurons (ORNs) are critical part to detect the sex pheromones. In genus Ostrinia, several pheromone receptors were functional analyzed in O. nubilalis and O. scapulalis bu...

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Autores principales: Liu, Wei, Jiang, Xing-chuan, Cao, Song, Yang, Bin, Wang, Gui-rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974041/
https://www.ncbi.nlm.nih.gov/pubmed/29875691
http://dx.doi.org/10.3389/fphys.2018.00591
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author Liu, Wei
Jiang, Xing-chuan
Cao, Song
Yang, Bin
Wang, Gui-rong
author_facet Liu, Wei
Jiang, Xing-chuan
Cao, Song
Yang, Bin
Wang, Gui-rong
author_sort Liu, Wei
collection PubMed
description Lepidopteran insects use sex pheromones for sexual communication. Pheromone receptors expressed on peripheral olfactory receptor neurons (ORNs) are critical part to detect the sex pheromones. In genus Ostrinia, several pheromone receptors were functional analyzed in O. nubilalis and O. scapulalis but the knowledge in O. furnacalis was rare. In this study, seven pheromone receptors were deorphanized by heterologous expression system of Xenopus oocytes. Functional types of sensilla trichoidea were classified by single sensillum recordings to interpret the response pattern of olfactory sensory neurons to Ostrinia pheromone components. OfurOR4 and OfurOR6 responded to the major sex pheromone Z/E12-14:OAc. OfurOR4 is the main receptor for both Z/E12-14:OAc and OfurOR6 mainly responded to E12-14:OAc. Functional differentiation of gene duplication were found between OfurOR5a and OfurOR5b. OfurOR5b showed a broad response to most of the pheromone components in O. furnacalis, whereas OfurOR5a was found without ligands. OfurOR7 showed a specific response to Z9-14:OAc and OfurOR8 mainly responded to Z11-14:OAc and E11-14:OAc. OfurOR3 did not respond to any pheromone components. Our results improved the current knowledge of pheromone reception in Ostrinia species which may contribute to speciation.
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spelling pubmed-59740412018-06-06 Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis Liu, Wei Jiang, Xing-chuan Cao, Song Yang, Bin Wang, Gui-rong Front Physiol Physiology Lepidopteran insects use sex pheromones for sexual communication. Pheromone receptors expressed on peripheral olfactory receptor neurons (ORNs) are critical part to detect the sex pheromones. In genus Ostrinia, several pheromone receptors were functional analyzed in O. nubilalis and O. scapulalis but the knowledge in O. furnacalis was rare. In this study, seven pheromone receptors were deorphanized by heterologous expression system of Xenopus oocytes. Functional types of sensilla trichoidea were classified by single sensillum recordings to interpret the response pattern of olfactory sensory neurons to Ostrinia pheromone components. OfurOR4 and OfurOR6 responded to the major sex pheromone Z/E12-14:OAc. OfurOR4 is the main receptor for both Z/E12-14:OAc and OfurOR6 mainly responded to E12-14:OAc. Functional differentiation of gene duplication were found between OfurOR5a and OfurOR5b. OfurOR5b showed a broad response to most of the pheromone components in O. furnacalis, whereas OfurOR5a was found without ligands. OfurOR7 showed a specific response to Z9-14:OAc and OfurOR8 mainly responded to Z11-14:OAc and E11-14:OAc. OfurOR3 did not respond to any pheromone components. Our results improved the current knowledge of pheromone reception in Ostrinia species which may contribute to speciation. Frontiers Media S.A. 2018-05-23 /pmc/articles/PMC5974041/ /pubmed/29875691 http://dx.doi.org/10.3389/fphys.2018.00591 Text en Copyright © 2018 Liu, Jiang, Cao, Yang and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Liu, Wei
Jiang, Xing-chuan
Cao, Song
Yang, Bin
Wang, Gui-rong
Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis
title Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis
title_full Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis
title_fullStr Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis
title_full_unstemmed Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis
title_short Functional Studies of Sex Pheromone Receptors in Asian Corn Borer Ostrinia furnacalis
title_sort functional studies of sex pheromone receptors in asian corn borer ostrinia furnacalis
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974041/
https://www.ncbi.nlm.nih.gov/pubmed/29875691
http://dx.doi.org/10.3389/fphys.2018.00591
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