A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages

The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15–30% of cell lines used in research ha...

Descripción completa

Detalles Bibliográficos
Autores principales: Evans, Elizabeth, Paillot, Romain, López-Álvarez, María Rocío
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974405/
https://www.ncbi.nlm.nih.gov/pubmed/29844485
http://dx.doi.org/10.1038/s41598-018-26512-3
_version_ 1783326812431974400
author Evans, Elizabeth
Paillot, Romain
López-Álvarez, María Rocío
author_facet Evans, Elizabeth
Paillot, Romain
López-Álvarez, María Rocío
author_sort Evans, Elizabeth
collection PubMed
description The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15–30% of cell lines used in research have been misidentified or cross-contaminated generating concerns about the results obtained from experiments that use them. Here we described how old aliquots of an equine macrophage cell line (e-CAS) stored at the Animal Health Trust did not contain equine cells but macrophages of murine origin (m-CAS).
format Online
Article
Text
id pubmed-5974405
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-59744052018-05-31 A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages Evans, Elizabeth Paillot, Romain López-Álvarez, María Rocío Sci Rep Article The 3Rs principles (Replacement, Reduction and Refinement) are focused on finding alternatives to the use of animals in research. In this regard, cell lines are popular and useful tools for the replacement of primary cells in in vitro studies. However, around 15–30% of cell lines used in research have been misidentified or cross-contaminated generating concerns about the results obtained from experiments that use them. Here we described how old aliquots of an equine macrophage cell line (e-CAS) stored at the Animal Health Trust did not contain equine cells but macrophages of murine origin (m-CAS). Nature Publishing Group UK 2018-05-29 /pmc/articles/PMC5974405/ /pubmed/29844485 http://dx.doi.org/10.1038/s41598-018-26512-3 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Evans, Elizabeth
Paillot, Romain
López-Álvarez, María Rocío
A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_full A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_fullStr A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_full_unstemmed A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_short A comprehensive analysis of e-CAS cell line reveals they are mouse macrophages
title_sort comprehensive analysis of e-cas cell line reveals they are mouse macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974405/
https://www.ncbi.nlm.nih.gov/pubmed/29844485
http://dx.doi.org/10.1038/s41598-018-26512-3
work_keys_str_mv AT evanselizabeth acomprehensiveanalysisofecascelllinerevealstheyaremousemacrophages
AT paillotromain acomprehensiveanalysisofecascelllinerevealstheyaremousemacrophages
AT lopezalvarezmariarocio acomprehensiveanalysisofecascelllinerevealstheyaremousemacrophages
AT evanselizabeth comprehensiveanalysisofecascelllinerevealstheyaremousemacrophages
AT paillotromain comprehensiveanalysisofecascelllinerevealstheyaremousemacrophages
AT lopezalvarezmariarocio comprehensiveanalysisofecascelllinerevealstheyaremousemacrophages

Ejemplares similares