Molluscan Shell Matrix Characterization by Preparative SDS-PAGE

The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral pha...

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Detalles Bibliográficos
Autor principal: Marin, Frederic
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2003
Materias:
Peer-Reviewed Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974769/
https://www.ncbi.nlm.nih.gov/pubmed/12806098
http://dx.doi.org/10.1100/tsw.2003.30
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author Marin, Frederic
author_facet Marin, Frederic
author_sort Marin, Frederic
collection PubMed
description The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated “blind” on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix.
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spelling pubmed-59747692018-06-10 Molluscan Shell Matrix Characterization by Preparative SDS-PAGE Marin, Frederic ScientificWorldJournal Peer-Reviewed Protocol The glycoproteinaceous constituents of molluscan shell matrices usually resist chromatographical fractionation. We describe a protocol that overcomes this difficulty and permits collection of a large amount of shell proteins for further in vitro characterization. After dissolution of the mineral phase, the glycoproteins are fractionated “blind” on a preparative electrophoresis. They are subsequently detected with a polyclonal antibody raised against the whole matrix. TheScientificWorldJOURNAL 2003-05-05 /pmc/articles/PMC5974769/ /pubmed/12806098 http://dx.doi.org/10.1100/tsw.2003.30 Text en Copyright © 2003 Frederic Marin. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Peer-Reviewed Protocol
Marin, Frederic
Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_full Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_fullStr Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_full_unstemmed Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_short Molluscan Shell Matrix Characterization by Preparative SDS-PAGE
title_sort molluscan shell matrix characterization by preparative sds-page
topic Peer-Reviewed Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974769/
https://www.ncbi.nlm.nih.gov/pubmed/12806098
http://dx.doi.org/10.1100/tsw.2003.30
work_keys_str_mv AT marinfrederic molluscanshellmatrixcharacterizationbypreparativesdspage

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