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Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E

Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last decade, the precise biochemical pathways involved...

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Autores principales: Anderson, Claire L., Williams, Gwyn T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: TheScientificWorldJOURNAL 2003
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974873/
https://www.ncbi.nlm.nih.gov/pubmed/12806120
http://dx.doi.org/10.1100/tsw.2003.07
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author Anderson, Claire L.
Williams, Gwyn T.
author_facet Anderson, Claire L.
Williams, Gwyn T.
author_sort Anderson, Claire L.
collection PubMed
description Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last decade, the precise biochemical pathways involved in apoptosis remain incomplete and potentially novel genes may, as yet, remain undiscovered. The hunt is therefore on to bridge the remaining gaps in our knowledge. Our contribution to this research effort utilises a functional cloning approach to isolate important regulatory genes involved in apoptosis. This mini-review focuses on the use and advantages of a retroviral expression cloning strategy and describes the isolation and identification of one such potential apoptosis regulatory gene, namely that encoding vacuolar ATPase subunit E.
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spelling pubmed-59748732018-06-10 Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E Anderson, Claire L. Williams, Gwyn T. ScientificWorldJournal Research Article Over the past 10-15 years there has been an explosion of interest in apoptosis. The delayed realisation that cell death is an essential part of life for any multicellular organism has meant that, despite the recent and rapid developments of the last decade, the precise biochemical pathways involved in apoptosis remain incomplete and potentially novel genes may, as yet, remain undiscovered. The hunt is therefore on to bridge the remaining gaps in our knowledge. Our contribution to this research effort utilises a functional cloning approach to isolate important regulatory genes involved in apoptosis. This mini-review focuses on the use and advantages of a retroviral expression cloning strategy and describes the isolation and identification of one such potential apoptosis regulatory gene, namely that encoding vacuolar ATPase subunit E. TheScientificWorldJOURNAL 2003-03-17 /pmc/articles/PMC5974873/ /pubmed/12806120 http://dx.doi.org/10.1100/tsw.2003.07 Text en Copyright © 2003 Claire L. Anderson and Gwyn T. Williams. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Anderson, Claire L.
Williams, Gwyn T.
Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E
title Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E
title_full Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E
title_fullStr Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E
title_full_unstemmed Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E
title_short Apoptosis Gene Hunting Using Retroviral Expression Cloning: Identification of Vacuolar ATPase Subunit E
title_sort apoptosis gene hunting using retroviral expression cloning: identification of vacuolar atpase subunit e
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5974873/
https://www.ncbi.nlm.nih.gov/pubmed/12806120
http://dx.doi.org/10.1100/tsw.2003.07
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