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The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis
BACKGROUND: Angiostrongylus cantonensis is a human zoonotic nematode parasite. Our previous studies found that PAS-5 and Galectin-1 (Gal-1) proteins of A. cantonensis could be strongly recognized by sera from mice infected with A. cantonensis. In this study, we further evaluated the potential roles...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5975691/ https://www.ncbi.nlm.nih.gov/pubmed/29843794 http://dx.doi.org/10.1186/s13071-018-2894-5 |
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author | Yan, Lan-Zhu Shi, Xiao-Meng Zu, Yan-Wen Shen, Yuan-Yuan Chen, Xi-Xi Zhao, Meng-Jing Li, Xing-Pan Yan, Bao-Long Huang, Hui-Cong |
author_facet | Yan, Lan-Zhu Shi, Xiao-Meng Zu, Yan-Wen Shen, Yuan-Yuan Chen, Xi-Xi Zhao, Meng-Jing Li, Xing-Pan Yan, Bao-Long Huang, Hui-Cong |
author_sort | Yan, Lan-Zhu |
collection | PubMed |
description | BACKGROUND: Angiostrongylus cantonensis is a human zoonotic nematode parasite. Our previous studies found that PAS-5 and Galectin-1 (Gal-1) proteins of A. cantonensis could be strongly recognized by sera from mice infected with A. cantonensis. In this study, we further evaluated the potential roles of these two proteins in the induction of immune response in mice. METHODS: Mice were immunized with recombinant PAS-5 or Gal-1 and then challenged with 30 infective A. cantonensis larvae following the last immunization. We then examined the infected mice for changes in serum antibodies and cytokines by ELISA, CD4(+) T cells and CD4(+)CD25(+)FoxP3(+) regulatory T cells (Tregs) by flow cytometry, and tissue damage severity by hematoxylin-eosin (H&E) staining. RESULTS: Compared with control mice, the PAS-5-immunized mice exhibited increased levels of serum antibodies and cytokines (except for IL-10) at different time points post-infection. PAS-5 immunization promoted significant proliferation of CD4(+) T cells, and caused more damage in the brain tissue. Vaccination with Gal-1 inhibited the production of antibodies (except for IgG1) and IFN-γ, but promoted the expression of IL-4 and IL-10. Gal-1 immunization results in significant increases in the levels of CD4(+)CD25(+)FoxP3(+) Tregs, and mild inflammatory changes. CONCLUSIONS: Taken together, our findings show that PAS-5 enhances, but Gal-1 inhibits the immune response in the early stage of A. cantonensis infections. |
format | Online Article Text |
id | pubmed-5975691 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-59756912018-05-31 The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis Yan, Lan-Zhu Shi, Xiao-Meng Zu, Yan-Wen Shen, Yuan-Yuan Chen, Xi-Xi Zhao, Meng-Jing Li, Xing-Pan Yan, Bao-Long Huang, Hui-Cong Parasit Vectors Research BACKGROUND: Angiostrongylus cantonensis is a human zoonotic nematode parasite. Our previous studies found that PAS-5 and Galectin-1 (Gal-1) proteins of A. cantonensis could be strongly recognized by sera from mice infected with A. cantonensis. In this study, we further evaluated the potential roles of these two proteins in the induction of immune response in mice. METHODS: Mice were immunized with recombinant PAS-5 or Gal-1 and then challenged with 30 infective A. cantonensis larvae following the last immunization. We then examined the infected mice for changes in serum antibodies and cytokines by ELISA, CD4(+) T cells and CD4(+)CD25(+)FoxP3(+) regulatory T cells (Tregs) by flow cytometry, and tissue damage severity by hematoxylin-eosin (H&E) staining. RESULTS: Compared with control mice, the PAS-5-immunized mice exhibited increased levels of serum antibodies and cytokines (except for IL-10) at different time points post-infection. PAS-5 immunization promoted significant proliferation of CD4(+) T cells, and caused more damage in the brain tissue. Vaccination with Gal-1 inhibited the production of antibodies (except for IgG1) and IFN-γ, but promoted the expression of IL-4 and IL-10. Gal-1 immunization results in significant increases in the levels of CD4(+)CD25(+)FoxP3(+) Tregs, and mild inflammatory changes. CONCLUSIONS: Taken together, our findings show that PAS-5 enhances, but Gal-1 inhibits the immune response in the early stage of A. cantonensis infections. BioMed Central 2018-05-29 /pmc/articles/PMC5975691/ /pubmed/29843794 http://dx.doi.org/10.1186/s13071-018-2894-5 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Yan, Lan-Zhu Shi, Xiao-Meng Zu, Yan-Wen Shen, Yuan-Yuan Chen, Xi-Xi Zhao, Meng-Jing Li, Xing-Pan Yan, Bao-Long Huang, Hui-Cong The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
title | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
title_full | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
title_fullStr | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
title_full_unstemmed | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
title_short | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
title_sort | opposite roles of pas-5 and galectin-1 in immune response during the early infection of angiostrongylus cantonensis |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5975691/ https://www.ncbi.nlm.nih.gov/pubmed/29843794 http://dx.doi.org/10.1186/s13071-018-2894-5 |
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