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Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues
Optical properties of biological tissues can be influenced by their temperature, thus affecting light transport inside the sample. This could potentially be exploited to deliver more photons inside large biological samples, when compared with experiments at room temperature, overcoming some of diffi...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976642/ https://www.ncbi.nlm.nih.gov/pubmed/29849076 http://dx.doi.org/10.1038/s41598-018-25465-x |
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author | Ghita, Adrian Matousek, Pavel Stone, Nick |
author_facet | Ghita, Adrian Matousek, Pavel Stone, Nick |
author_sort | Ghita, Adrian |
collection | PubMed |
description | Optical properties of biological tissues can be influenced by their temperature, thus affecting light transport inside the sample. This could potentially be exploited to deliver more photons inside large biological samples, when compared with experiments at room temperature, overcoming some of difficulties due to highly scattering nature of the tissue. Here we report a change in light transmitted inside biological tissue with temperature elevation from 20 to 40 °C, indicating a considerable enhancement of photons collected by the detector in transmission geometry. The measurement of Raman signals in porcine tissue samples, as large as 40 mm in thickness, indicates a considerable increase in signal ranging from 1.3 to 2 fold, subject to biological variability. The enhancements observed are ascribed to phase transitions of lipids in biological samples. This indicates that: 1) experiments performed on tissue at room temperature can lead to an underestimation of signals that would be obtained at depth in the body in vivo and 2) that experiments at room temperature could be modified to increase detection limits by elevating the temperature of the material of interest. |
format | Online Article Text |
id | pubmed-5976642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-59766422018-05-31 Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues Ghita, Adrian Matousek, Pavel Stone, Nick Sci Rep Article Optical properties of biological tissues can be influenced by their temperature, thus affecting light transport inside the sample. This could potentially be exploited to deliver more photons inside large biological samples, when compared with experiments at room temperature, overcoming some of difficulties due to highly scattering nature of the tissue. Here we report a change in light transmitted inside biological tissue with temperature elevation from 20 to 40 °C, indicating a considerable enhancement of photons collected by the detector in transmission geometry. The measurement of Raman signals in porcine tissue samples, as large as 40 mm in thickness, indicates a considerable increase in signal ranging from 1.3 to 2 fold, subject to biological variability. The enhancements observed are ascribed to phase transitions of lipids in biological samples. This indicates that: 1) experiments performed on tissue at room temperature can lead to an underestimation of signals that would be obtained at depth in the body in vivo and 2) that experiments at room temperature could be modified to increase detection limits by elevating the temperature of the material of interest. Nature Publishing Group UK 2018-05-30 /pmc/articles/PMC5976642/ /pubmed/29849076 http://dx.doi.org/10.1038/s41598-018-25465-x Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ghita, Adrian Matousek, Pavel Stone, Nick Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues |
title | Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues |
title_full | Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues |
title_fullStr | Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues |
title_full_unstemmed | Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues |
title_short | Sensitivity of Transmission Raman Spectroscopy Signals to Temperature of Biological Tissues |
title_sort | sensitivity of transmission raman spectroscopy signals to temperature of biological tissues |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976642/ https://www.ncbi.nlm.nih.gov/pubmed/29849076 http://dx.doi.org/10.1038/s41598-018-25465-x |
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