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Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary
For a better understanding of epigenetic regulation of cell differentiation, it is important to analyze DNA methylation at a specific site. In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976889/ https://www.ncbi.nlm.nih.gov/pubmed/29867282 http://dx.doi.org/10.1267/ahc.17039 |
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author | Liu, Jin Zhang, Wenchang Wu, Zhiren Dai, Lei Koji, Takehiko |
author_facet | Liu, Jin Zhang, Wenchang Wu, Zhiren Dai, Lei Koji, Takehiko |
author_sort | Liu, Jin |
collection | PubMed |
description | For a better understanding of epigenetic regulation of cell differentiation, it is important to analyze DNA methylation at a specific site. In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles compared to that of primary and secondary follicles. Interestingly, TUNEL-positive granulosa cells, which were frequent in mammalian folliculogenesis, became markedly Hpa II-reactive and Sau3A I-reactive, indicating that the CCGG and GATCG sites may be preferentially demethylated during apoptosis. |
format | Online Article Text |
id | pubmed-5976889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY |
record_format | MEDLINE/PubMed |
spelling | pubmed-59768892018-06-04 Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary Liu, Jin Zhang, Wenchang Wu, Zhiren Dai, Lei Koji, Takehiko Acta Histochem Cytochem Regular Article For a better understanding of epigenetic regulation of cell differentiation, it is important to analyze DNA methylation at a specific site. In this study, we examined changes in the methylation level of CCGG and GATCG sites during mouse folliculogenesis in paraffin-embedded sections of mouse ovaries. For the purpose, we used a new method, histo endonuclease-linked detection of methylation sites of DNA (HELMET), designed to detect methylation sites of DNA with a specific sequence in a tissue section. Unlike the global level of DNA methylation, which was no change in immunohistochemical staining of 5-methylcytosine throughout folliculogenesis, we found that there were hypermethylation of CCGG and GATCG sites in most of the granulosa cells of tertiary follicles compared to that of primary and secondary follicles. Interestingly, TUNEL-positive granulosa cells, which were frequent in mammalian folliculogenesis, became markedly Hpa II-reactive and Sau3A I-reactive, indicating that the CCGG and GATCG sites may be preferentially demethylated during apoptosis. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2018-04-27 2018-04-24 /pmc/articles/PMC5976889/ /pubmed/29867282 http://dx.doi.org/10.1267/ahc.17039 Text en 2018 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Liu, Jin Zhang, Wenchang Wu, Zhiren Dai, Lei Koji, Takehiko Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary |
title | Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary |
title_full | Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary |
title_fullStr | Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary |
title_full_unstemmed | Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary |
title_short | Changes in DNA Methylation of Oocytes and Granulosa Cells Assessed by HELMET during Folliculogenesis in Mouse Ovary |
title_sort | changes in dna methylation of oocytes and granulosa cells assessed by helmet during folliculogenesis in mouse ovary |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976889/ https://www.ncbi.nlm.nih.gov/pubmed/29867282 http://dx.doi.org/10.1267/ahc.17039 |
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