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Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files

AIM: This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. METHODS: Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmen...

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Autores principales: Heggendorn, Fabiano Luiz, Fraga, Aline Guerra Manssour, Ferreira, Dennis de Carvalho, Gonçalves, Lucio Souza, Lione, Viviane de Oliveira Freitas, Lutterbach, Márcia Teresa Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976933/
https://www.ncbi.nlm.nih.gov/pubmed/29861730
http://dx.doi.org/10.1155/2018/8303450
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author Heggendorn, Fabiano Luiz
Fraga, Aline Guerra Manssour
Ferreira, Dennis de Carvalho
Gonçalves, Lucio Souza
Lione, Viviane de Oliveira Freitas
Lutterbach, Márcia Teresa Soares
author_facet Heggendorn, Fabiano Luiz
Fraga, Aline Guerra Manssour
Ferreira, Dennis de Carvalho
Gonçalves, Lucio Souza
Lione, Viviane de Oliveira Freitas
Lutterbach, Márcia Teresa Soares
author_sort Heggendorn, Fabiano Luiz
collection PubMed
description AIM: This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. METHODS: Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmental) were inoculated into the culture media (Postgate C culture medium or modified Postgate E culture medium). The biocorrosive potential of these bacteria will be an important component of a biopharmaceutical under development called BACCOR. Afterwards, four used endodontic files (UEFs) were separately inoculated into a specific culture media for 445 days at 30°C in an incubator. The four UEFs were placed in a scanning electron microscope (SEM) and analyzed by the energy-dispersive X-ray spectrometry (EDS). RESULTS: The confocal laser scanning microscopic images indicate the presence of biofilm in the four samples. The SEM and SEM-EDS revealed the presence of rough, irregular structures adhering along the metallic surface of the used endodontic files, suggesting a mature calcified biofilm with a high concentration of Ca, P, C, and S. CONCLUSION: The formation of SRB biofilms on used endodontic files shows characteristics that may contribute to the biocorrosion of these files, and the results may also provide complementary data for a biopharmaceutical, which is still under development to assist in the removal of fractured endodontic files inside root channels.
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spelling pubmed-59769332018-06-03 Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files Heggendorn, Fabiano Luiz Fraga, Aline Guerra Manssour Ferreira, Dennis de Carvalho Gonçalves, Lucio Souza Lione, Viviane de Oliveira Freitas Lutterbach, Márcia Teresa Soares Int J Dent Research Article AIM: This study describes the biofilm formation and the corrosive capacity of sulfate-reducing bacteria (SRB) on the metallic structure of used endodontic files. METHODS: Sulfate-reducing bacteria (SRB) (Desulfovibrio desulfuricans oral and Desulfovibrio fairfieldensis or D. desulfuricans environmental) were inoculated into the culture media (Postgate C culture medium or modified Postgate E culture medium). The biocorrosive potential of these bacteria will be an important component of a biopharmaceutical under development called BACCOR. Afterwards, four used endodontic files (UEFs) were separately inoculated into a specific culture media for 445 days at 30°C in an incubator. The four UEFs were placed in a scanning electron microscope (SEM) and analyzed by the energy-dispersive X-ray spectrometry (EDS). RESULTS: The confocal laser scanning microscopic images indicate the presence of biofilm in the four samples. The SEM and SEM-EDS revealed the presence of rough, irregular structures adhering along the metallic surface of the used endodontic files, suggesting a mature calcified biofilm with a high concentration of Ca, P, C, and S. CONCLUSION: The formation of SRB biofilms on used endodontic files shows characteristics that may contribute to the biocorrosion of these files, and the results may also provide complementary data for a biopharmaceutical, which is still under development to assist in the removal of fractured endodontic files inside root channels. Hindawi 2018-05-10 /pmc/articles/PMC5976933/ /pubmed/29861730 http://dx.doi.org/10.1155/2018/8303450 Text en Copyright © 2018 Fabiano Luiz Heggendorn et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Heggendorn, Fabiano Luiz
Fraga, Aline Guerra Manssour
Ferreira, Dennis de Carvalho
Gonçalves, Lucio Souza
Lione, Viviane de Oliveira Freitas
Lutterbach, Márcia Teresa Soares
Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
title Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
title_full Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
title_fullStr Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
title_full_unstemmed Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
title_short Sulfate-Reducing Bacteria: Biofilm Formation and Corrosive Activity in Endodontic Files
title_sort sulfate-reducing bacteria: biofilm formation and corrosive activity in endodontic files
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976933/
https://www.ncbi.nlm.nih.gov/pubmed/29861730
http://dx.doi.org/10.1155/2018/8303450
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