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Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane

The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepar...

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Autores principales: Lee, Shyh-Yuan, Wu, Sheng-Chien, Chen, Hsuan, Tsai, Lo-Lin, Tzeng, Jy-Jiunn, Lin, Chih-Hsin, Lin, Yuan-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976978/
https://www.ncbi.nlm.nih.gov/pubmed/29862262
http://dx.doi.org/10.1155/2018/3240571
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author Lee, Shyh-Yuan
Wu, Sheng-Chien
Chen, Hsuan
Tsai, Lo-Lin
Tzeng, Jy-Jiunn
Lin, Chih-Hsin
Lin, Yuan-Min
author_facet Lee, Shyh-Yuan
Wu, Sheng-Chien
Chen, Hsuan
Tsai, Lo-Lin
Tzeng, Jy-Jiunn
Lin, Chih-Hsin
Lin, Yuan-Min
author_sort Lee, Shyh-Yuan
collection PubMed
description The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL) diol with 1,6-hexamethylene diisocyanate (HDI) at different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. The chemical, mechanical, and thermal properties of the synthesized polymers were determined using NMR, FTIR, GPC, DSC, and tensile tester. The PCL and polyurethanes were fabricated as nanofiber membranes by electrospinning, and their mechanical properties and SEM morphology were also investigated. In vitro tests, including WST-1 assay, SEM of cells, and phalloidin cytoskeleton staining, were also performed. It was shown that electrospun membranes made of PCL and PCL-HDI-PEG (2 : 3 : 1) possessed tensile strength of 19.84 MPa and 11.72 MPa and elongation at break of 627% and 362%, respectively. These numbers are equivalent or higher than most of the commercially available collagen and PTFE membrane. As a result, these membranes may have potential for future GBR applications.
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spelling pubmed-59769782018-06-03 Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane Lee, Shyh-Yuan Wu, Sheng-Chien Chen, Hsuan Tsai, Lo-Lin Tzeng, Jy-Jiunn Lin, Chih-Hsin Lin, Yuan-Min Biomed Res Int Research Article The aim of this research is to synthesize polycaprolactone-based polyurethanes (PCL-based PUs) that can be further used for the fabrication of guided bone regeneration (GBR) membranes with higher tensile strength and elongation at break than collagen and PTFE membranes. The PCL-based PUs were prepared by the polymerization of polycaprolactone (PCL) diol with 1,6-hexamethylene diisocyanate (HDI) at different ratios using either polyethylene glycol (PEG) or ethylenediamine (EDA) as chain extenders. The chemical, mechanical, and thermal properties of the synthesized polymers were determined using NMR, FTIR, GPC, DSC, and tensile tester. The PCL and polyurethanes were fabricated as nanofiber membranes by electrospinning, and their mechanical properties and SEM morphology were also investigated. In vitro tests, including WST-1 assay, SEM of cells, and phalloidin cytoskeleton staining, were also performed. It was shown that electrospun membranes made of PCL and PCL-HDI-PEG (2 : 3 : 1) possessed tensile strength of 19.84 MPa and 11.72 MPa and elongation at break of 627% and 362%, respectively. These numbers are equivalent or higher than most of the commercially available collagen and PTFE membrane. As a result, these membranes may have potential for future GBR applications. Hindawi 2018-05-15 /pmc/articles/PMC5976978/ /pubmed/29862262 http://dx.doi.org/10.1155/2018/3240571 Text en Copyright © 2018 Shyh-Yuan Lee et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Shyh-Yuan
Wu, Sheng-Chien
Chen, Hsuan
Tsai, Lo-Lin
Tzeng, Jy-Jiunn
Lin, Chih-Hsin
Lin, Yuan-Min
Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
title Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
title_full Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
title_fullStr Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
title_full_unstemmed Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
title_short Synthesis and Characterization of Polycaprolactone-Based Polyurethanes for the Fabrication of Elastic Guided Bone Regeneration Membrane
title_sort synthesis and characterization of polycaprolactone-based polyurethanes for the fabrication of elastic guided bone regeneration membrane
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976978/
https://www.ncbi.nlm.nih.gov/pubmed/29862262
http://dx.doi.org/10.1155/2018/3240571
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