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Sensing of Vascular Permeability in Inflamed Vessel of Live Animal

Increase in vascular permeability is a conclusive response in the progress of inflammation. Under controlled conditions, leukocytes are known to migrate across the vascular barriers to the sites of inflammation without severe vascular rupture. However, when inflammatory state becomes excessive, the...

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Autores principales: Park, Sang A, Jeong, Soi, Choe, Young Ho, Hyun, Young-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976979/
https://www.ncbi.nlm.nih.gov/pubmed/29862122
http://dx.doi.org/10.1155/2018/5797152
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author Park, Sang A
Jeong, Soi
Choe, Young Ho
Hyun, Young-Min
author_facet Park, Sang A
Jeong, Soi
Choe, Young Ho
Hyun, Young-Min
author_sort Park, Sang A
collection PubMed
description Increase in vascular permeability is a conclusive response in the progress of inflammation. Under controlled conditions, leukocytes are known to migrate across the vascular barriers to the sites of inflammation without severe vascular rupture. However, when inflammatory state becomes excessive, the leakage of blood components may occur and can be lethal. Basically, vascular permeability can be analyzed based on the intensity of blood outflow. To evaluate the amount and rate of leakage in live mice, we performed cremaster muscle exteriorization to visualize blood flow and neutrophil migration. Using two-photon intravital microscopy of the exteriorized cremaster muscle venules, we found that vascular barrier function is transiently and locally disrupted in the early stage of inflammatory condition induced by N-formylmethionyl-leucyl-phenylalanine (fMLP). Measurement of the concentration of intravenously (i.v.) injected Texas Red dextran inside and outside the vessels resulted in clear visualization of real-time increases in transient and local vascular permeability increase in real-time manner. We successfully demonstrated repeated leakage from a target site on a blood vessel in association with increasing severity of inflammation. Therefore, compared to other methods, two-photon intravital microscopy more accurately visualizes and quantifies vascular permeability even in a small part of blood vessels in live animals in real time.
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spelling pubmed-59769792018-06-03 Sensing of Vascular Permeability in Inflamed Vessel of Live Animal Park, Sang A Jeong, Soi Choe, Young Ho Hyun, Young-Min J Anal Methods Chem Research Article Increase in vascular permeability is a conclusive response in the progress of inflammation. Under controlled conditions, leukocytes are known to migrate across the vascular barriers to the sites of inflammation without severe vascular rupture. However, when inflammatory state becomes excessive, the leakage of blood components may occur and can be lethal. Basically, vascular permeability can be analyzed based on the intensity of blood outflow. To evaluate the amount and rate of leakage in live mice, we performed cremaster muscle exteriorization to visualize blood flow and neutrophil migration. Using two-photon intravital microscopy of the exteriorized cremaster muscle venules, we found that vascular barrier function is transiently and locally disrupted in the early stage of inflammatory condition induced by N-formylmethionyl-leucyl-phenylalanine (fMLP). Measurement of the concentration of intravenously (i.v.) injected Texas Red dextran inside and outside the vessels resulted in clear visualization of real-time increases in transient and local vascular permeability increase in real-time manner. We successfully demonstrated repeated leakage from a target site on a blood vessel in association with increasing severity of inflammation. Therefore, compared to other methods, two-photon intravital microscopy more accurately visualizes and quantifies vascular permeability even in a small part of blood vessels in live animals in real time. Hindawi 2018-05-15 /pmc/articles/PMC5976979/ /pubmed/29862122 http://dx.doi.org/10.1155/2018/5797152 Text en Copyright © 2018 Sang A Park et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Park, Sang A
Jeong, Soi
Choe, Young Ho
Hyun, Young-Min
Sensing of Vascular Permeability in Inflamed Vessel of Live Animal
title Sensing of Vascular Permeability in Inflamed Vessel of Live Animal
title_full Sensing of Vascular Permeability in Inflamed Vessel of Live Animal
title_fullStr Sensing of Vascular Permeability in Inflamed Vessel of Live Animal
title_full_unstemmed Sensing of Vascular Permeability in Inflamed Vessel of Live Animal
title_short Sensing of Vascular Permeability in Inflamed Vessel of Live Animal
title_sort sensing of vascular permeability in inflamed vessel of live animal
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5976979/
https://www.ncbi.nlm.nih.gov/pubmed/29862122
http://dx.doi.org/10.1155/2018/5797152
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