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Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli

Atypical enteropathogenic Escherichia coli are capable to form biofilm on biotic and abiotic surfaces, regardless of the adherence pattern displayed. Several E. coli mechanisms are regulated by Quorum sensing (QS), including virulence factors and biofilm formation. Quorum sensing is a signaling syst...

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Autores principales: Culler, Hebert F., Couto, Samuel C. F., Higa, Juliana S., Ruiz, Renato M., J. Yang, Min, Bueris, Vanessa, Franzolin, Marcia R., Sircili, Marcelo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977193/
https://www.ncbi.nlm.nih.gov/pubmed/29762495
http://dx.doi.org/10.3390/genes9050253
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author Culler, Hebert F.
Couto, Samuel C. F.
Higa, Juliana S.
Ruiz, Renato M.
J. Yang, Min
Bueris, Vanessa
Franzolin, Marcia R.
Sircili, Marcelo P.
author_facet Culler, Hebert F.
Couto, Samuel C. F.
Higa, Juliana S.
Ruiz, Renato M.
J. Yang, Min
Bueris, Vanessa
Franzolin, Marcia R.
Sircili, Marcelo P.
author_sort Culler, Hebert F.
collection PubMed
description Atypical enteropathogenic Escherichia coli are capable to form biofilm on biotic and abiotic surfaces, regardless of the adherence pattern displayed. Several E. coli mechanisms are regulated by Quorum sensing (QS), including virulence factors and biofilm formation. Quorum sensing is a signaling system that confers bacteria with the ability to respond to chemical molecules known as autoinducers. Suppressor of division inhibitor (SdiA) is a QS receptor present in atypical enteropathogenic E. coli (aEPEC) that detects acyl homoserine lactone (AHL) type autoinducers. However, these bacteria do not encode an AHL synthase, but they are capable of sensing AHL molecules produced by other species, establishing an inter-species bacterial communication. In this study, we performed experiments to evaluate pellicle, ring-like structure and biofilm formation on wild type, sdiA mutants and complemented strains. We also evaluated the transcription of genes involved in different stages of biofilm formation, such as bcsA, csgA, csgD, fliC and fimA. The sdiA mutants were capable of forming thicker biofilm structures and showed increased motility when compared to wild type and complemented strains. Moreover, they also showed denser pellicles and ring-like structures. Quantitative real-time PCR (qRT-PCR) analysis demonstrated increased csgA, csgD and fliC transcription on mutant strains. Biofilm formation, as well as csgD, csgA and fimA transcription decreased on wild type strains by the addition of AHL. These results indicate that SdiA participates on the regulation of these phenotypes in aEPEC and that AHL addition enhances the repressor effect of this receptor on the transcription of biofilm and motility related genes.
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spelling pubmed-59771932018-05-31 Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli Culler, Hebert F. Couto, Samuel C. F. Higa, Juliana S. Ruiz, Renato M. J. Yang, Min Bueris, Vanessa Franzolin, Marcia R. Sircili, Marcelo P. Genes (Basel) Article Atypical enteropathogenic Escherichia coli are capable to form biofilm on biotic and abiotic surfaces, regardless of the adherence pattern displayed. Several E. coli mechanisms are regulated by Quorum sensing (QS), including virulence factors and biofilm formation. Quorum sensing is a signaling system that confers bacteria with the ability to respond to chemical molecules known as autoinducers. Suppressor of division inhibitor (SdiA) is a QS receptor present in atypical enteropathogenic E. coli (aEPEC) that detects acyl homoserine lactone (AHL) type autoinducers. However, these bacteria do not encode an AHL synthase, but they are capable of sensing AHL molecules produced by other species, establishing an inter-species bacterial communication. In this study, we performed experiments to evaluate pellicle, ring-like structure and biofilm formation on wild type, sdiA mutants and complemented strains. We also evaluated the transcription of genes involved in different stages of biofilm formation, such as bcsA, csgA, csgD, fliC and fimA. The sdiA mutants were capable of forming thicker biofilm structures and showed increased motility when compared to wild type and complemented strains. Moreover, they also showed denser pellicles and ring-like structures. Quantitative real-time PCR (qRT-PCR) analysis demonstrated increased csgA, csgD and fliC transcription on mutant strains. Biofilm formation, as well as csgD, csgA and fimA transcription decreased on wild type strains by the addition of AHL. These results indicate that SdiA participates on the regulation of these phenotypes in aEPEC and that AHL addition enhances the repressor effect of this receptor on the transcription of biofilm and motility related genes. MDPI 2018-05-15 /pmc/articles/PMC5977193/ /pubmed/29762495 http://dx.doi.org/10.3390/genes9050253 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Culler, Hebert F.
Couto, Samuel C. F.
Higa, Juliana S.
Ruiz, Renato M.
J. Yang, Min
Bueris, Vanessa
Franzolin, Marcia R.
Sircili, Marcelo P.
Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli
title Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli
title_full Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli
title_fullStr Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli
title_full_unstemmed Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli
title_short Role of SdiA on Biofilm Formation by Atypical Enteropathogenic Escherichia coli
title_sort role of sdia on biofilm formation by atypical enteropathogenic escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977193/
https://www.ncbi.nlm.nih.gov/pubmed/29762495
http://dx.doi.org/10.3390/genes9050253
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