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Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome
Recombination is one of the determinants of genetic diversity in the foot-and-mouth disease virus (FMDV). FMDV sequences have a mosaic structure caused by extensive intra- and inter-serotype recombination, with the exception of the capsid-encoding region. While these genome-wide patterns of broad-sc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977214/ https://www.ncbi.nlm.nih.gov/pubmed/29693634 http://dx.doi.org/10.3390/v10050221 |
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author | Ferretti, Luca Di Nardo, Antonello Singer, Benjamin Lasecka-Dykes, Lidia Logan, Grace Wright, Caroline F. Pérez-Martín, Eva King, Donald P. Tuthill, Tobias J. Ribeca, Paolo |
author_facet | Ferretti, Luca Di Nardo, Antonello Singer, Benjamin Lasecka-Dykes, Lidia Logan, Grace Wright, Caroline F. Pérez-Martín, Eva King, Donald P. Tuthill, Tobias J. Ribeca, Paolo |
author_sort | Ferretti, Luca |
collection | PubMed |
description | Recombination is one of the determinants of genetic diversity in the foot-and-mouth disease virus (FMDV). FMDV sequences have a mosaic structure caused by extensive intra- and inter-serotype recombination, with the exception of the capsid-encoding region. While these genome-wide patterns of broad-scale recombination are well studied, not much is known about the patterns of recombination that may exist within infected hosts. In addition, detection of recombination among viruses evolving at the within-host level is challenging due to the similarity of the sequences and the limitations in differentiating recombination from point mutations. Here, we present the first analysis of recombination events between closely related FMDV sequences occurring within buffalo hosts. The detection of these events was made possible by the occurrence of co-infection of two viral swarms with about 1% nucleotide divergence. We found more than 15 recombination events, unequally distributed across eight samples from different animals. The distribution of these events along the FMDV genome was neither uniform nor related to the phylogenetic distribution of recombination breakpoints, suggesting a mismatch between within-host evolutionary pressures and long-term selection for infectivity and transmissibility. |
format | Online Article Text |
id | pubmed-5977214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59772142018-06-01 Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome Ferretti, Luca Di Nardo, Antonello Singer, Benjamin Lasecka-Dykes, Lidia Logan, Grace Wright, Caroline F. Pérez-Martín, Eva King, Donald P. Tuthill, Tobias J. Ribeca, Paolo Viruses Article Recombination is one of the determinants of genetic diversity in the foot-and-mouth disease virus (FMDV). FMDV sequences have a mosaic structure caused by extensive intra- and inter-serotype recombination, with the exception of the capsid-encoding region. While these genome-wide patterns of broad-scale recombination are well studied, not much is known about the patterns of recombination that may exist within infected hosts. In addition, detection of recombination among viruses evolving at the within-host level is challenging due to the similarity of the sequences and the limitations in differentiating recombination from point mutations. Here, we present the first analysis of recombination events between closely related FMDV sequences occurring within buffalo hosts. The detection of these events was made possible by the occurrence of co-infection of two viral swarms with about 1% nucleotide divergence. We found more than 15 recombination events, unequally distributed across eight samples from different animals. The distribution of these events along the FMDV genome was neither uniform nor related to the phylogenetic distribution of recombination breakpoints, suggesting a mismatch between within-host evolutionary pressures and long-term selection for infectivity and transmissibility. MDPI 2018-04-25 /pmc/articles/PMC5977214/ /pubmed/29693634 http://dx.doi.org/10.3390/v10050221 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ferretti, Luca Di Nardo, Antonello Singer, Benjamin Lasecka-Dykes, Lidia Logan, Grace Wright, Caroline F. Pérez-Martín, Eva King, Donald P. Tuthill, Tobias J. Ribeca, Paolo Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome |
title | Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome |
title_full | Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome |
title_fullStr | Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome |
title_full_unstemmed | Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome |
title_short | Within-Host Recombination in the Foot-and-Mouth Disease Virus Genome |
title_sort | within-host recombination in the foot-and-mouth disease virus genome |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977214/ https://www.ncbi.nlm.nih.gov/pubmed/29693634 http://dx.doi.org/10.3390/v10050221 |
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