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Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections
Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3′-terminus and 5′-ter...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977253/ https://www.ncbi.nlm.nih.gov/pubmed/29762514 http://dx.doi.org/10.3390/v10050260 |
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author | James, Delano Phelan, James Sanderson, Daniel |
author_facet | James, Delano Phelan, James Sanderson, Daniel |
author_sort | James, Delano |
collection | PubMed |
description | Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3′-terminus and 5′-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R enzyme treatments, with denaturation at 95 °C preceding each digestion. Evidence was obtained indicating that there are circular forms of BCLCaV RNA-2 and RNA-3. |
format | Online Article Text |
id | pubmed-5977253 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59772532018-06-01 Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections James, Delano Phelan, James Sanderson, Daniel Viruses Article Blackcurrant leaf chlorosis associated virus (BCLCaV) was detected recently by next-generation sequencing (NGS) and a new and distinct species in the genus Idaeovirus was proposed. Analysis of NGS-derived paired-end reads revealed the existence of bridge reads encompassing the 3′-terminus and 5′-terminus of RNA-2 or RNA-3 of BCLCaV. The full RNA-2 or RNA-3 could be amplified using outward facing or abutting primers; also, RNA-2/RNA-3 could be detected even after three consecutive RNase R enzyme treatments, with denaturation at 95 °C preceding each digestion. Evidence was obtained indicating that there are circular forms of BCLCaV RNA-2 and RNA-3. MDPI 2018-05-15 /pmc/articles/PMC5977253/ /pubmed/29762514 http://dx.doi.org/10.3390/v10050260 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article James, Delano Phelan, James Sanderson, Daniel Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections |
title | Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections |
title_full | Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections |
title_fullStr | Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections |
title_full_unstemmed | Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections |
title_short | Blackcurrant Leaf Chlorosis Associated Virus: Evidence of the Presence of Circular RNA during Infections |
title_sort | blackcurrant leaf chlorosis associated virus: evidence of the presence of circular rna during infections |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977253/ https://www.ncbi.nlm.nih.gov/pubmed/29762514 http://dx.doi.org/10.3390/v10050260 |
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