Cargando…
Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line
Mitochondria are multifunctional organelles that participate in numerous processes in response to viral infection, but they are also a target for viruses. The aim of this study was to define subcellular events leading to alterations in mitochondrial morphology and function during infection with ectr...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977259/ https://www.ncbi.nlm.nih.gov/pubmed/29772718 http://dx.doi.org/10.3390/v10050266 |
_version_ | 1783327341810810880 |
---|---|
author | Gregorczyk, Karolina P. Wyżewski, Zbigniew Szczepanowska, Joanna Toka, Felix N. Mielcarska, Matylda B. Bossowska-Nowicka, Magdalena Gieryńska, Małgorzata Boratyńska-Jasińska, Anna Struzik, Justyna Niemiałtowski, Marek G. Szulc-Dąbrowska, Lidia |
author_facet | Gregorczyk, Karolina P. Wyżewski, Zbigniew Szczepanowska, Joanna Toka, Felix N. Mielcarska, Matylda B. Bossowska-Nowicka, Magdalena Gieryńska, Małgorzata Boratyńska-Jasińska, Anna Struzik, Justyna Niemiałtowski, Marek G. Szulc-Dąbrowska, Lidia |
author_sort | Gregorczyk, Karolina P. |
collection | PubMed |
description | Mitochondria are multifunctional organelles that participate in numerous processes in response to viral infection, but they are also a target for viruses. The aim of this study was to define subcellular events leading to alterations in mitochondrial morphology and function during infection with ectromelia virus (ECTV). We used two different cell lines and a combination of immunofluorescence techniques, confocal and electron microscopy, and flow cytometry to address subcellular changes following infection. Early in infection of L929 fibroblasts and RAW 264.7 macrophages, mitochondria gathered around viral factories. Later, the mitochondrial network became fragmented, forming punctate mitochondria that co-localized with the progeny virions. ECTV-co-localized mitochondria associated with the cytoskeleton components. Mitochondrial membrane potential, mitochondrial fission–fusion, mitochondrial mass, and generation of reactive oxygen species (ROS) were severely altered later in ECTV infection leading to damage of mitochondria. These results suggest an important role of mitochondria in supplying energy for virus replication and morphogenesis. Presumably, mitochondria participate in transport of viral particles inside and outside of the cell and/or they are a source of membranes for viral envelope formation. We speculate that the observed changes in the mitochondrial network organization and physiology in ECTV-infected cells provide suitable conditions for viral replication and morphogenesis. |
format | Online Article Text |
id | pubmed-5977259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59772592018-06-01 Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line Gregorczyk, Karolina P. Wyżewski, Zbigniew Szczepanowska, Joanna Toka, Felix N. Mielcarska, Matylda B. Bossowska-Nowicka, Magdalena Gieryńska, Małgorzata Boratyńska-Jasińska, Anna Struzik, Justyna Niemiałtowski, Marek G. Szulc-Dąbrowska, Lidia Viruses Article Mitochondria are multifunctional organelles that participate in numerous processes in response to viral infection, but they are also a target for viruses. The aim of this study was to define subcellular events leading to alterations in mitochondrial morphology and function during infection with ectromelia virus (ECTV). We used two different cell lines and a combination of immunofluorescence techniques, confocal and electron microscopy, and flow cytometry to address subcellular changes following infection. Early in infection of L929 fibroblasts and RAW 264.7 macrophages, mitochondria gathered around viral factories. Later, the mitochondrial network became fragmented, forming punctate mitochondria that co-localized with the progeny virions. ECTV-co-localized mitochondria associated with the cytoskeleton components. Mitochondrial membrane potential, mitochondrial fission–fusion, mitochondrial mass, and generation of reactive oxygen species (ROS) were severely altered later in ECTV infection leading to damage of mitochondria. These results suggest an important role of mitochondria in supplying energy for virus replication and morphogenesis. Presumably, mitochondria participate in transport of viral particles inside and outside of the cell and/or they are a source of membranes for viral envelope formation. We speculate that the observed changes in the mitochondrial network organization and physiology in ECTV-infected cells provide suitable conditions for viral replication and morphogenesis. MDPI 2018-05-16 /pmc/articles/PMC5977259/ /pubmed/29772718 http://dx.doi.org/10.3390/v10050266 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gregorczyk, Karolina P. Wyżewski, Zbigniew Szczepanowska, Joanna Toka, Felix N. Mielcarska, Matylda B. Bossowska-Nowicka, Magdalena Gieryńska, Małgorzata Boratyńska-Jasińska, Anna Struzik, Justyna Niemiałtowski, Marek G. Szulc-Dąbrowska, Lidia Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line |
title | Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line |
title_full | Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line |
title_fullStr | Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line |
title_full_unstemmed | Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line |
title_short | Ectromelia Virus Affects Mitochondrial Network Morphology, Distribution, and Physiology in Murine Fibroblasts and Macrophage Cell Line |
title_sort | ectromelia virus affects mitochondrial network morphology, distribution, and physiology in murine fibroblasts and macrophage cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977259/ https://www.ncbi.nlm.nih.gov/pubmed/29772718 http://dx.doi.org/10.3390/v10050266 |
work_keys_str_mv | AT gregorczykkarolinap ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT wyzewskizbigniew ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT szczepanowskajoanna ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT tokafelixn ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT mielcarskamatyldab ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT bossowskanowickamagdalena ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT gierynskamałgorzata ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT boratynskajasinskaanna ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT struzikjustyna ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT niemiałtowskimarekg ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline AT szulcdabrowskalidia ectromeliavirusaffectsmitochondrialnetworkmorphologydistributionandphysiologyinmurinefibroblastsandmacrophagecellline |