Live-Cell Imaging of Early Steps of Single HIV-1 Infection

Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protei...

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Detalles Bibliográficos
Autores principales: Francis, Ashwanth C., Melikyan, Gregory B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977268/
https://www.ncbi.nlm.nih.gov/pubmed/29783762
http://dx.doi.org/10.3390/v10050275
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author Francis, Ashwanth C.
Melikyan, Gregory B.
author_facet Francis, Ashwanth C.
Melikyan, Gregory B.
author_sort Francis, Ashwanth C.
collection PubMed
description Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection.
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spelling pubmed-59772682018-06-01 Live-Cell Imaging of Early Steps of Single HIV-1 Infection Francis, Ashwanth C. Melikyan, Gregory B. Viruses Review Live-cell imaging of single HIV-1 entry offers a unique opportunity to delineate the spatio-temporal regulation of infection. Novel virus labeling and imaging approaches enable the visualization of key steps of HIV-1 entry leading to nuclear import, integration into the host genome, and viral protein expression. Here, we discuss single virus imaging strategies, focusing on live-cell imaging of single virus fusion and productive uncoating that culminates in HIV-1 infection. MDPI 2018-05-19 /pmc/articles/PMC5977268/ /pubmed/29783762 http://dx.doi.org/10.3390/v10050275 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Francis, Ashwanth C.
Melikyan, Gregory B.
Live-Cell Imaging of Early Steps of Single HIV-1 Infection
title Live-Cell Imaging of Early Steps of Single HIV-1 Infection
title_full Live-Cell Imaging of Early Steps of Single HIV-1 Infection
title_fullStr Live-Cell Imaging of Early Steps of Single HIV-1 Infection
title_full_unstemmed Live-Cell Imaging of Early Steps of Single HIV-1 Infection
title_short Live-Cell Imaging of Early Steps of Single HIV-1 Infection
title_sort live-cell imaging of early steps of single hiv-1 infection
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977268/
https://www.ncbi.nlm.nih.gov/pubmed/29783762
http://dx.doi.org/10.3390/v10050275
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