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Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments
This study aimed to treat hepatocellular ailments with biologically prepared silver nanoparticle (AgNPs). AgNPs were formulated using Morus alba leaf extract and their synthesis and characterization were determined by UV–visible spectroscopy, Transmission Electron Microscope (TEM), Scanning Electron...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977380/ https://www.ncbi.nlm.nih.gov/pubmed/29854602 http://dx.doi.org/10.1016/j.toxrep.2018.02.013 |
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author | Singh, Asha Dar, Mohd Yaqoob Joshi, Beenu Sharma, Bhawna Shrivastava, Sadhana Shukla, Sangeeta |
author_facet | Singh, Asha Dar, Mohd Yaqoob Joshi, Beenu Sharma, Bhawna Shrivastava, Sadhana Shukla, Sangeeta |
author_sort | Singh, Asha |
collection | PubMed |
description | This study aimed to treat hepatocellular ailments with biologically prepared silver nanoparticle (AgNPs). AgNPs were formulated using Morus alba leaf extract and their synthesis and characterization were determined by UV–visible spectroscopy, Transmission Electron Microscope (TEM), Scanning Electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and Zeta analysis. In vitro studies on HepG2 cell lines for cytotoxic effect and in vivo studies in a rat model for hepatoprotective effect were carried out using biologically prepared AgNPs as curing agents. Dose response cytotoxicity on hepatic cancer (HepG2) cells was confirmed by 3-(4, 5-dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium (MTT) assay. The inhibitory concentrations (IC(50)) were found to be 20 μg/mL and 80 μg/mL for AgNPs and M. alba leaf extract respectively against HepG2 cells at 24 h incubation. In addition, hepatotoxicity in Wistar rats (180 ± 10 g) was induced by intraperitoneal injection of N-nitrosodiethylamine (NDEA) and were treated with different doses of AgNPs (25, 50, 100 μg/kg). NDEA administration showed a significant rise in the biochemical parameters whereas the levels of enzymic antioxidants were decreased. Obtained results revealed that the elevated levels of Liver Function Test (LFTs) biomarkers were significantly reversed and the antioxidant levels were significantly recouped towards normal after the conjoint treatment of AgNPs in a dose-dependent manner. Thus green synthesized AgNPs showed a promising curing effect on hepatocellular ailments. |
format | Online Article Text |
id | pubmed-5977380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-59773802018-05-31 Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments Singh, Asha Dar, Mohd Yaqoob Joshi, Beenu Sharma, Bhawna Shrivastava, Sadhana Shukla, Sangeeta Toxicol Rep Article This study aimed to treat hepatocellular ailments with biologically prepared silver nanoparticle (AgNPs). AgNPs were formulated using Morus alba leaf extract and their synthesis and characterization were determined by UV–visible spectroscopy, Transmission Electron Microscope (TEM), Scanning Electron microscope (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR) and Zeta analysis. In vitro studies on HepG2 cell lines for cytotoxic effect and in vivo studies in a rat model for hepatoprotective effect were carried out using biologically prepared AgNPs as curing agents. Dose response cytotoxicity on hepatic cancer (HepG2) cells was confirmed by 3-(4, 5-dimethyl thiazole-2-yl)-2, 5-diphenyl tetrazolium (MTT) assay. The inhibitory concentrations (IC(50)) were found to be 20 μg/mL and 80 μg/mL for AgNPs and M. alba leaf extract respectively against HepG2 cells at 24 h incubation. In addition, hepatotoxicity in Wistar rats (180 ± 10 g) was induced by intraperitoneal injection of N-nitrosodiethylamine (NDEA) and were treated with different doses of AgNPs (25, 50, 100 μg/kg). NDEA administration showed a significant rise in the biochemical parameters whereas the levels of enzymic antioxidants were decreased. Obtained results revealed that the elevated levels of Liver Function Test (LFTs) biomarkers were significantly reversed and the antioxidant levels were significantly recouped towards normal after the conjoint treatment of AgNPs in a dose-dependent manner. Thus green synthesized AgNPs showed a promising curing effect on hepatocellular ailments. Elsevier 2018-03-01 /pmc/articles/PMC5977380/ /pubmed/29854602 http://dx.doi.org/10.1016/j.toxrep.2018.02.013 Text en © 2018 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Singh, Asha Dar, Mohd Yaqoob Joshi, Beenu Sharma, Bhawna Shrivastava, Sadhana Shukla, Sangeeta Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments |
title | Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments |
title_full | Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments |
title_fullStr | Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments |
title_full_unstemmed | Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments |
title_short | Phytofabrication of Silver nanoparticles: Novel Drug to overcome hepatocellular ailments |
title_sort | phytofabrication of silver nanoparticles: novel drug to overcome hepatocellular ailments |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977380/ https://www.ncbi.nlm.nih.gov/pubmed/29854602 http://dx.doi.org/10.1016/j.toxrep.2018.02.013 |
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