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Septic cardiomyopathy: The value of lactoferrin and CD15 as specific markers to corroborate a definitive diagnosis
Current scientific consensus about the physiopathology in the progression from severe sepsis to septic shock and death focuses on myocardial contractile dysfunction. Nevertheless, objective parameters to establish a pathological correlate of a fatal outcome are lacking; then a cause of death due to...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977426/ https://www.ncbi.nlm.nih.gov/pubmed/29809052 http://dx.doi.org/10.1177/2058738418776526 |
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author | Galassi, Andrea Turatello, Liliana De Salvia, Alessandra Neri, Margherita Turillazzi, Emanuela La Russa, Raffaele Viola, Rocco V Frati, Paola Fineschi, Vittorio |
author_facet | Galassi, Andrea Turatello, Liliana De Salvia, Alessandra Neri, Margherita Turillazzi, Emanuela La Russa, Raffaele Viola, Rocco V Frati, Paola Fineschi, Vittorio |
author_sort | Galassi, Andrea |
collection | PubMed |
description | Current scientific consensus about the physiopathology in the progression from severe sepsis to septic shock and death focuses on myocardial contractile dysfunction. Nevertheless, objective parameters to establish a pathological correlate of a fatal outcome are lacking; then a cause of death due to sepsis can remain an unsolved problem. We first reviewed all death cases recorded at our institutions during the period from 2007 until 2015. Then, we conducted a retrospective study of a selected autopsy series of people who had received “sepsis” as cause of death. Two pathologists re-examined the heart sections while the most suitable myocardial sample for each case was stained for immunohistochemistry with antibodies targeted for specific inflammatory-related molecules. We used specific antibodies for the following markers: alpha-smooth muscle actin (alpha-SMA); fibronectin; matrix metallopeptidase 9 (MMP-9); intercellular adhesion molecule 1 (ICAM-1); caspase-3; lactoferrin (LF); cluster differentiation 15 (CD15). The statistical significance of differences was assessed using student’s t-test for unpaired data or non-parametric Mann–Whitney or Wilcoxon tests for skewed variables or one-way analysis of variance and post hoc Scheffe’s test for continuous variables and Pearson’s χ(2)-test for discrete variables. Linear regression analysis was used to determine the presence of a correlation between continuous variables. At our institutions, 2220 deaths have been recorded during the period study. Sepsis accounted as a cause of death for the 20% of total. We finally enrolled 56 cases; of these, only 20 were positive for microbiological analysis. At histological examination, clear inflammation was detectable in the 32% of cases; otherwise, immunohistochemical reaction showed a positive reaction for LF and CD15 in more than a half cases (56%). We still ignore all the underlying mechanisms of sepsis and all its pathophysiological connections with cardiac metabolism; in this sense, we aim to corroborate the diagnostic value of anti-LF and anti-CD15 staining for the post-mortem detection of myocardial inflammation. |
format | Online Article Text |
id | pubmed-5977426 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-59774262019-03-14 Septic cardiomyopathy: The value of lactoferrin and CD15 as specific markers to corroborate a definitive diagnosis Galassi, Andrea Turatello, Liliana De Salvia, Alessandra Neri, Margherita Turillazzi, Emanuela La Russa, Raffaele Viola, Rocco V Frati, Paola Fineschi, Vittorio Int J Immunopathol Pharmacol Letter to the Editor Current scientific consensus about the physiopathology in the progression from severe sepsis to septic shock and death focuses on myocardial contractile dysfunction. Nevertheless, objective parameters to establish a pathological correlate of a fatal outcome are lacking; then a cause of death due to sepsis can remain an unsolved problem. We first reviewed all death cases recorded at our institutions during the period from 2007 until 2015. Then, we conducted a retrospective study of a selected autopsy series of people who had received “sepsis” as cause of death. Two pathologists re-examined the heart sections while the most suitable myocardial sample for each case was stained for immunohistochemistry with antibodies targeted for specific inflammatory-related molecules. We used specific antibodies for the following markers: alpha-smooth muscle actin (alpha-SMA); fibronectin; matrix metallopeptidase 9 (MMP-9); intercellular adhesion molecule 1 (ICAM-1); caspase-3; lactoferrin (LF); cluster differentiation 15 (CD15). The statistical significance of differences was assessed using student’s t-test for unpaired data or non-parametric Mann–Whitney or Wilcoxon tests for skewed variables or one-way analysis of variance and post hoc Scheffe’s test for continuous variables and Pearson’s χ(2)-test for discrete variables. Linear regression analysis was used to determine the presence of a correlation between continuous variables. At our institutions, 2220 deaths have been recorded during the period study. Sepsis accounted as a cause of death for the 20% of total. We finally enrolled 56 cases; of these, only 20 were positive for microbiological analysis. At histological examination, clear inflammation was detectable in the 32% of cases; otherwise, immunohistochemical reaction showed a positive reaction for LF and CD15 in more than a half cases (56%). We still ignore all the underlying mechanisms of sepsis and all its pathophysiological connections with cardiac metabolism; in this sense, we aim to corroborate the diagnostic value of anti-LF and anti-CD15 staining for the post-mortem detection of myocardial inflammation. SAGE Publications 2018-05-29 /pmc/articles/PMC5977426/ /pubmed/29809052 http://dx.doi.org/10.1177/2058738418776526 Text en © The Author(s) 2018 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages(https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Letter to the Editor Galassi, Andrea Turatello, Liliana De Salvia, Alessandra Neri, Margherita Turillazzi, Emanuela La Russa, Raffaele Viola, Rocco V Frati, Paola Fineschi, Vittorio Septic cardiomyopathy: The value of lactoferrin and CD15 as specific markers to corroborate a definitive diagnosis |
title | Septic cardiomyopathy: The value of lactoferrin and CD15 as specific
markers to corroborate a definitive diagnosis |
title_full | Septic cardiomyopathy: The value of lactoferrin and CD15 as specific
markers to corroborate a definitive diagnosis |
title_fullStr | Septic cardiomyopathy: The value of lactoferrin and CD15 as specific
markers to corroborate a definitive diagnosis |
title_full_unstemmed | Septic cardiomyopathy: The value of lactoferrin and CD15 as specific
markers to corroborate a definitive diagnosis |
title_short | Septic cardiomyopathy: The value of lactoferrin and CD15 as specific
markers to corroborate a definitive diagnosis |
title_sort | septic cardiomyopathy: the value of lactoferrin and cd15 as specific
markers to corroborate a definitive diagnosis |
topic | Letter to the Editor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977426/ https://www.ncbi.nlm.nih.gov/pubmed/29809052 http://dx.doi.org/10.1177/2058738418776526 |
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