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Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case

BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS: We obtained multiple of tissues from a fatal case with SFTS, incl...

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Autores principales: Li, Shibo, Li, Yang, Wang, Qiujing, Yu, Xuewen, Liu, Miaomiao, Xie, Haibo, Qian, Liyong, Ye, Ling, Yang, Zhejuan, Zhang, Jianjing, Zhu, Huimin, Zhang, Wenhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977472/
https://www.ncbi.nlm.nih.gov/pubmed/29848330
http://dx.doi.org/10.1186/s12985-018-1006-7
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author Li, Shibo
Li, Yang
Wang, Qiujing
Yu, Xuewen
Liu, Miaomiao
Xie, Haibo
Qian, Liyong
Ye, Ling
Yang, Zhejuan
Zhang, Jianjing
Zhu, Huimin
Zhang, Wenhong
author_facet Li, Shibo
Li, Yang
Wang, Qiujing
Yu, Xuewen
Liu, Miaomiao
Xie, Haibo
Qian, Liyong
Ye, Ling
Yang, Zhejuan
Zhang, Jianjing
Zhu, Huimin
Zhang, Wenhong
author_sort Li, Shibo
collection PubMed
description BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS: We obtained multiple of tissues from a fatal case with SFTS, including blood, lungs, kidneys, heart, and spleen. The blood samples were used to isolate the causative agent for detection of viral RNA and further expression of recombinant viral protein as primary antibody. Immunohistochemistry of the heart, lungs, spleen and kidneys was used to characterize the viral antigen in tissue sections. RESULTS: A 79-year-old man, together with his wife, was admitted because of fever. Both patients were diagnosed with SFTS by the positive SFTSV RNA in the blood. The gentleman died of multiple organ failure 8 days after hospitalization. However, his wife recovered and was discharged. Immunohistochemistry indicated that SFTSV antigens were present in all studied organs including the heart, kidney, lung and spleen, of which the spleen presented with the highest amount of SFTSV antigens. The kidney was next while the heart and lungs showed lower amount of SFTSV antigens. CONCLUSIONS: SFTSV can direct infect multiple organs, resulting in multiple organ failure and ultimately in an unfavorable outcome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1006-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-59774722018-05-31 Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case Li, Shibo Li, Yang Wang, Qiujing Yu, Xuewen Liu, Miaomiao Xie, Haibo Qian, Liyong Ye, Ling Yang, Zhejuan Zhang, Jianjing Zhu, Huimin Zhang, Wenhong Virol J Research BACKGROUND: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS: We obtained multiple of tissues from a fatal case with SFTS, including blood, lungs, kidneys, heart, and spleen. The blood samples were used to isolate the causative agent for detection of viral RNA and further expression of recombinant viral protein as primary antibody. Immunohistochemistry of the heart, lungs, spleen and kidneys was used to characterize the viral antigen in tissue sections. RESULTS: A 79-year-old man, together with his wife, was admitted because of fever. Both patients were diagnosed with SFTS by the positive SFTSV RNA in the blood. The gentleman died of multiple organ failure 8 days after hospitalization. However, his wife recovered and was discharged. Immunohistochemistry indicated that SFTSV antigens were present in all studied organs including the heart, kidney, lung and spleen, of which the spleen presented with the highest amount of SFTSV antigens. The kidney was next while the heart and lungs showed lower amount of SFTSV antigens. CONCLUSIONS: SFTSV can direct infect multiple organs, resulting in multiple organ failure and ultimately in an unfavorable outcome. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12985-018-1006-7) contains supplementary material, which is available to authorized users. BioMed Central 2018-05-30 /pmc/articles/PMC5977472/ /pubmed/29848330 http://dx.doi.org/10.1186/s12985-018-1006-7 Text en © The Author(s). 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Li, Shibo
Li, Yang
Wang, Qiujing
Yu, Xuewen
Liu, Miaomiao
Xie, Haibo
Qian, Liyong
Ye, Ling
Yang, Zhejuan
Zhang, Jianjing
Zhu, Huimin
Zhang, Wenhong
Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
title Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
title_full Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
title_fullStr Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
title_full_unstemmed Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
title_short Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
title_sort multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977472/
https://www.ncbi.nlm.nih.gov/pubmed/29848330
http://dx.doi.org/10.1186/s12985-018-1006-7
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