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Entering an era of dynamic structural biology…
A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX). They apply their method to resolve in near atomic detail the cleavage and inactiva...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977480/ https://www.ncbi.nlm.nih.gov/pubmed/29848334 http://dx.doi.org/10.1186/s12915-018-0533-4 |
| _version_ | 1783327387211005952 |
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| author | Orville, Allen M. |
| author_facet | Orville, Allen M. |
| author_sort | Orville, Allen M. |
| collection | PubMed |
| description | A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX). They apply their method to resolve in near atomic detail the cleavage and inactivation of the antibiotic ceftriaxone by a β-lactamase enzyme from Mycobacterium tuberculosis. Their work demonstrates the general applicability of time-resolved crystallography, from which dynamic structures, at atomic resolution, can be obtained. See research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0524-5. |
| format | Online Article Text |
| id | pubmed-5977480 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2018 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-59774802018-06-06 Entering an era of dynamic structural biology… Orville, Allen M. BMC Biol Commentary A recent paper in BMC Biology presents a general method for mix-and-inject serial crystallography, to facilitate the visualization of enzyme intermediates via time-resolved serial femtosecond crystallography (tr-SFX). They apply their method to resolve in near atomic detail the cleavage and inactivation of the antibiotic ceftriaxone by a β-lactamase enzyme from Mycobacterium tuberculosis. Their work demonstrates the general applicability of time-resolved crystallography, from which dynamic structures, at atomic resolution, can be obtained. See research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-018-0524-5. BioMed Central 2018-05-31 /pmc/articles/PMC5977480/ /pubmed/29848334 http://dx.doi.org/10.1186/s12915-018-0533-4 Text en © Orville et al. 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
| spellingShingle | Commentary Orville, Allen M. Entering an era of dynamic structural biology… |
| title | Entering an era of dynamic structural biology… |
| title_full | Entering an era of dynamic structural biology… |
| title_fullStr | Entering an era of dynamic structural biology… |
| title_full_unstemmed | Entering an era of dynamic structural biology… |
| title_short | Entering an era of dynamic structural biology… |
| title_sort | entering an era of dynamic structural biology… |
| topic | Commentary |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5977480/ https://www.ncbi.nlm.nih.gov/pubmed/29848334 http://dx.doi.org/10.1186/s12915-018-0533-4 |
| work_keys_str_mv | AT orvilleallenm enteringaneraofdynamicstructuralbiology |