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Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data

High-throughput amplicon sequencing (HTAS) of conserved DNA regions is a powerful technique to characterize microbial communities. Recently, spike-in mock communities have been used to measure accuracy of sequencing platforms and data analysis pipelines. To assess the ability of sequencing platforms...

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Detalles Bibliográficos
Autores principales: Palmer, Jonathan M., Jusino, Michelle A., Banik, Mark T., Lindner, Daniel L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978393/
https://www.ncbi.nlm.nih.gov/pubmed/29868296
http://dx.doi.org/10.7717/peerj.4925