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Non-biological synthetic spike-in controls and the AMPtk software pipeline improve mycobiome data
High-throughput amplicon sequencing (HTAS) of conserved DNA regions is a powerful technique to characterize microbial communities. Recently, spike-in mock communities have been used to measure accuracy of sequencing platforms and data analysis pipelines. To assess the ability of sequencing platforms...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5978393/ https://www.ncbi.nlm.nih.gov/pubmed/29868296 http://dx.doi.org/10.7717/peerj.4925 |