Cargando…
Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins
Mitochondrial responses under drought within Brassica genus are poorly understood. The main goal of this study was to investigate mitochondrial biogenesis of three cauliflower (Brassica oleracea var. botrytis) cultivars with varying drought tolerance. Diverse quantitative changes (decreases in abund...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979313/ https://www.ncbi.nlm.nih.gov/pubmed/29642585 http://dx.doi.org/10.3390/ijms19041130 |
_version_ | 1783327666291605504 |
---|---|
author | Rurek, Michał Czołpińska, Magdalena Pawłowski, Tomasz Andrzej Staszak, Aleksandra Maria Nowak, Witold Krzesiński, Włodzimierz Spiżewski, Tomasz |
author_facet | Rurek, Michał Czołpińska, Magdalena Pawłowski, Tomasz Andrzej Staszak, Aleksandra Maria Nowak, Witold Krzesiński, Włodzimierz Spiżewski, Tomasz |
author_sort | Rurek, Michał |
collection | PubMed |
description | Mitochondrial responses under drought within Brassica genus are poorly understood. The main goal of this study was to investigate mitochondrial biogenesis of three cauliflower (Brassica oleracea var. botrytis) cultivars with varying drought tolerance. Diverse quantitative changes (decreases in abundance mostly) in the mitochondrial proteome were assessed by two-dimensional gel electrophoresis (2D PAGE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Respiratory (e.g., complex II, IV (CII, CIV) and ATP synthase subunits), transporter (including diverse porin isoforms) and matrix multifunctional proteins (e.g., components of RNA editing machinery) were diversely affected in their abundance under two drought levels. Western immunoassays showed additional cultivar-specific responses of selected mitochondrial proteins. Dehydrin-related tryptic peptides (found in several 2D spots) immunopositive with dehydrin-specific antisera highlighted the relevance of mitochondrial dehydrin-like proteins for the drought response. The abundance of selected mRNAs participating in drought response was also determined. We conclude that mitochondrial biogenesis was strongly, but diversely affected in various cauliflower cultivars, and associated with drought tolerance at the proteomic and functional levels. However, discussed alternative oxidase (AOX) regulation at the RNA and protein level were largely uncoordinated due to the altered availability of transcripts for translation, mRNA/ribosome interactions, and/or miRNA impact on transcript abundance and translation. |
format | Online Article Text |
id | pubmed-5979313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-59793132018-06-10 Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins Rurek, Michał Czołpińska, Magdalena Pawłowski, Tomasz Andrzej Staszak, Aleksandra Maria Nowak, Witold Krzesiński, Włodzimierz Spiżewski, Tomasz Int J Mol Sci Article Mitochondrial responses under drought within Brassica genus are poorly understood. The main goal of this study was to investigate mitochondrial biogenesis of three cauliflower (Brassica oleracea var. botrytis) cultivars with varying drought tolerance. Diverse quantitative changes (decreases in abundance mostly) in the mitochondrial proteome were assessed by two-dimensional gel electrophoresis (2D PAGE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Respiratory (e.g., complex II, IV (CII, CIV) and ATP synthase subunits), transporter (including diverse porin isoforms) and matrix multifunctional proteins (e.g., components of RNA editing machinery) were diversely affected in their abundance under two drought levels. Western immunoassays showed additional cultivar-specific responses of selected mitochondrial proteins. Dehydrin-related tryptic peptides (found in several 2D spots) immunopositive with dehydrin-specific antisera highlighted the relevance of mitochondrial dehydrin-like proteins for the drought response. The abundance of selected mRNAs participating in drought response was also determined. We conclude that mitochondrial biogenesis was strongly, but diversely affected in various cauliflower cultivars, and associated with drought tolerance at the proteomic and functional levels. However, discussed alternative oxidase (AOX) regulation at the RNA and protein level were largely uncoordinated due to the altered availability of transcripts for translation, mRNA/ribosome interactions, and/or miRNA impact on transcript abundance and translation. MDPI 2018-04-10 /pmc/articles/PMC5979313/ /pubmed/29642585 http://dx.doi.org/10.3390/ijms19041130 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Rurek, Michał Czołpińska, Magdalena Pawłowski, Tomasz Andrzej Staszak, Aleksandra Maria Nowak, Witold Krzesiński, Włodzimierz Spiżewski, Tomasz Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins |
title | Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins |
title_full | Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins |
title_fullStr | Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins |
title_full_unstemmed | Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins |
title_short | Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins |
title_sort | mitochondrial biogenesis in diverse cauliflower cultivars under mild and severe drought. impaired coordination of selected transcript and proteomic responses, and regulation of various multifunctional proteins |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979313/ https://www.ncbi.nlm.nih.gov/pubmed/29642585 http://dx.doi.org/10.3390/ijms19041130 |
work_keys_str_mv | AT rurekmichał mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins AT czołpinskamagdalena mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins AT pawłowskitomaszandrzej mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins AT staszakaleksandramaria mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins AT nowakwitold mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins AT krzesinskiwłodzimierz mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins AT spizewskitomasz mitochondrialbiogenesisindiversecauliflowercultivarsundermildandseveredroughtimpairedcoordinationofselectedtranscriptandproteomicresponsesandregulationofvariousmultifunctionalproteins |