Localization Microscopy of Actin Cytoskeleton in Human Platelets

Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Mor...

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Autores principales: Mayr, Sandra, Hauser, Fabian, Peterbauer, Anja, Tauscher, Andreas, Naderer, Christoph, Axmann, Markus, Plochberger, Birgit, Jacak, Jaroslaw
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979344/
https://www.ncbi.nlm.nih.gov/pubmed/29641438
http://dx.doi.org/10.3390/ijms19041150
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author Mayr, Sandra
Hauser, Fabian
Peterbauer, Anja
Tauscher, Andreas
Naderer, Christoph
Axmann, Markus
Plochberger, Birgit
Jacak, Jaroslaw
author_facet Mayr, Sandra
Hauser, Fabian
Peterbauer, Anja
Tauscher, Andreas
Naderer, Christoph
Axmann, Markus
Plochberger, Birgit
Jacak, Jaroslaw
author_sort Mayr, Sandra
collection PubMed
description Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm.
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spelling pubmed-59793442018-06-10 Localization Microscopy of Actin Cytoskeleton in Human Platelets Mayr, Sandra Hauser, Fabian Peterbauer, Anja Tauscher, Andreas Naderer, Christoph Axmann, Markus Plochberger, Birgit Jacak, Jaroslaw Int J Mol Sci Communication Here, we measure the actin cytoskeleton arrangement of different morphological states of human platelets using a new protocol for photo-switching of rhodamine class fluorophores. A new medium composition was established for imaging the cytoskeleton using Alexa Fluor 488 conjugated to phalloidin. Morphological states of platelets bound to a glass substrate are visualized and quantified by two-dimensional localization microscopy at nanoscopic resolution. Marker-less drift correction yields localization of individual Alexa 488 conjugated to phalloidin with a positional accuracy of 12 nm. MDPI 2018-04-11 /pmc/articles/PMC5979344/ /pubmed/29641438 http://dx.doi.org/10.3390/ijms19041150 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mayr, Sandra
Hauser, Fabian
Peterbauer, Anja
Tauscher, Andreas
Naderer, Christoph
Axmann, Markus
Plochberger, Birgit
Jacak, Jaroslaw
Localization Microscopy of Actin Cytoskeleton in Human Platelets
title Localization Microscopy of Actin Cytoskeleton in Human Platelets
title_full Localization Microscopy of Actin Cytoskeleton in Human Platelets
title_fullStr Localization Microscopy of Actin Cytoskeleton in Human Platelets
title_full_unstemmed Localization Microscopy of Actin Cytoskeleton in Human Platelets
title_short Localization Microscopy of Actin Cytoskeleton in Human Platelets
title_sort localization microscopy of actin cytoskeleton in human platelets
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979344/
https://www.ncbi.nlm.nih.gov/pubmed/29641438
http://dx.doi.org/10.3390/ijms19041150
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