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Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers

Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren’s syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure o...

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Autores principales: Sfakis, Lauren, Kamaldinov, Tim, Khmaladze, Alexander, Hosseini, Zeinab F., Nelson, Deirdre A., Larsen, Melinda, Castracane, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979364/
https://www.ncbi.nlm.nih.gov/pubmed/29596382
http://dx.doi.org/10.3390/ijms19041031
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author Sfakis, Lauren
Kamaldinov, Tim
Khmaladze, Alexander
Hosseini, Zeinab F.
Nelson, Deirdre A.
Larsen, Melinda
Castracane, James
author_facet Sfakis, Lauren
Kamaldinov, Tim
Khmaladze, Alexander
Hosseini, Zeinab F.
Nelson, Deirdre A.
Larsen, Melinda
Castracane, James
author_sort Sfakis, Lauren
collection PubMed
description Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren’s syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure of cells to interact with scaffolds and of the single cell types that are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber scaffolds have been used for in vitro growth of epithelial cells, PLGA has low compliance and cells do not penetrate the scaffolds. Using a core-shell electrospinning technique, we incorporated poly (glycerol sebacate) (PGS) into PLGA scaffolds to increase the compliance and decrease hydrophobicity. PGS/PLGA scaffolds promoted epithelial cell penetration into the scaffold and apical localization of tight junction proteins, which is necessary for epithelial cell function. Additionally, co-culture of the salivary epithelial cells with NIH3T3 mesenchymal cells on PGS/PLGA scaffolds facilitated epithelial tissue reorganization and apical localization of tight junction proteins significantly more than in the absence of the mesenchyme. These data demonstrate the applicability of PGS/PLGA nanofibers for epithelial cell self-organization and facilitation of co-culture cell interactions that promote tissue self-organization in vitro.
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spelling pubmed-59793642018-06-10 Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers Sfakis, Lauren Kamaldinov, Tim Khmaladze, Alexander Hosseini, Zeinab F. Nelson, Deirdre A. Larsen, Melinda Castracane, James Int J Mol Sci Article Engineering salivary glands is of interest due to the damaging effects of radiation therapy and the autoimmune disease Sjögren’s syndrome on salivary gland function. One of the current problems in tissue engineering is that in vitro studies often fail to predict in vivo regeneration due to failure of cells to interact with scaffolds and of the single cell types that are typically used for these studies. Although poly (lactic co glycolic acid) (PLGA) nanofiber scaffolds have been used for in vitro growth of epithelial cells, PLGA has low compliance and cells do not penetrate the scaffolds. Using a core-shell electrospinning technique, we incorporated poly (glycerol sebacate) (PGS) into PLGA scaffolds to increase the compliance and decrease hydrophobicity. PGS/PLGA scaffolds promoted epithelial cell penetration into the scaffold and apical localization of tight junction proteins, which is necessary for epithelial cell function. Additionally, co-culture of the salivary epithelial cells with NIH3T3 mesenchymal cells on PGS/PLGA scaffolds facilitated epithelial tissue reorganization and apical localization of tight junction proteins significantly more than in the absence of the mesenchyme. These data demonstrate the applicability of PGS/PLGA nanofibers for epithelial cell self-organization and facilitation of co-culture cell interactions that promote tissue self-organization in vitro. MDPI 2018-03-29 /pmc/articles/PMC5979364/ /pubmed/29596382 http://dx.doi.org/10.3390/ijms19041031 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sfakis, Lauren
Kamaldinov, Tim
Khmaladze, Alexander
Hosseini, Zeinab F.
Nelson, Deirdre A.
Larsen, Melinda
Castracane, James
Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers
title Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers
title_full Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers
title_fullStr Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers
title_full_unstemmed Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers
title_short Mesenchymal Cells Affect Salivary Epithelial Cell Morphology on PGS/PLGA Core/Shell Nanofibers
title_sort mesenchymal cells affect salivary epithelial cell morphology on pgs/plga core/shell nanofibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979364/
https://www.ncbi.nlm.nih.gov/pubmed/29596382
http://dx.doi.org/10.3390/ijms19041031
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