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Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation

Cooling reduces the ischemia/reperfusion (I/R) injury seen in sudden cardiac arrest (SCA) by decreasing the burst of reactive oxygen species (ROS). Its cardioprotection is diminished when delay in reaching the target temperature occurs. Baicalein, a flavonoid derived from the root of Scutellaria bai...

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Autores principales: Shao, Zuohui, Chen, Sy-Jou, Zhu, Xiangdong, Lee, Chunpei, Huang, Hsien-Hao, Meliton, Angelo, Li, Changqing, Vanden Hoek, Terry L., Li, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979521/
https://www.ncbi.nlm.nih.gov/pubmed/29587364
http://dx.doi.org/10.3390/ijms19040973
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author Shao, Zuohui
Chen, Sy-Jou
Zhu, Xiangdong
Lee, Chunpei
Huang, Hsien-Hao
Meliton, Angelo
Li, Changqing
Vanden Hoek, Terry L.
Li, Jing
author_facet Shao, Zuohui
Chen, Sy-Jou
Zhu, Xiangdong
Lee, Chunpei
Huang, Hsien-Hao
Meliton, Angelo
Li, Changqing
Vanden Hoek, Terry L.
Li, Jing
author_sort Shao, Zuohui
collection PubMed
description Cooling reduces the ischemia/reperfusion (I/R) injury seen in sudden cardiac arrest (SCA) by decreasing the burst of reactive oxygen species (ROS). Its cardioprotection is diminished when delay in reaching the target temperature occurs. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis Georgi, possesses antioxidant properties. Therefore, we hypothesized that baicalein can rescue cooling cardioprotection when cooling is delayed. Two murine cardiomyocyte models, an I/R model (90 min ischemia/3 h reperfusion) and stunning model (30 min ischemia/90 min reperfusion), were used to assess cell survival and contractility, respectively. Cooling (32 °C) was initiated either during ischemia or during reperfusion. Cell viability and ROS generation were measured. Cell contractility was evaluated by real-time phase-contrast imaging. Our results showed that cooling reduced cell death and ROS generation, and this effect was diminished when cooling was delayed. Baicalein (25 µM), given either at the start of reperfusion or start of cooling, resulted in a comparable reduction of cell death and ROS production. Baicalein improved phospholamban phosphorylation, contractility recovery, and cell survival. These effects were Akt-dependent. In addition, no synergistic effect was observed with the combined treatments of cooling and baicalein. Our data suggest that baicalein may serve as a novel adjunct therapeutic strategy for SCA resuscitation.
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spelling pubmed-59795212018-06-10 Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation Shao, Zuohui Chen, Sy-Jou Zhu, Xiangdong Lee, Chunpei Huang, Hsien-Hao Meliton, Angelo Li, Changqing Vanden Hoek, Terry L. Li, Jing Int J Mol Sci Article Cooling reduces the ischemia/reperfusion (I/R) injury seen in sudden cardiac arrest (SCA) by decreasing the burst of reactive oxygen species (ROS). Its cardioprotection is diminished when delay in reaching the target temperature occurs. Baicalein, a flavonoid derived from the root of Scutellaria baicalensis Georgi, possesses antioxidant properties. Therefore, we hypothesized that baicalein can rescue cooling cardioprotection when cooling is delayed. Two murine cardiomyocyte models, an I/R model (90 min ischemia/3 h reperfusion) and stunning model (30 min ischemia/90 min reperfusion), were used to assess cell survival and contractility, respectively. Cooling (32 °C) was initiated either during ischemia or during reperfusion. Cell viability and ROS generation were measured. Cell contractility was evaluated by real-time phase-contrast imaging. Our results showed that cooling reduced cell death and ROS generation, and this effect was diminished when cooling was delayed. Baicalein (25 µM), given either at the start of reperfusion or start of cooling, resulted in a comparable reduction of cell death and ROS production. Baicalein improved phospholamban phosphorylation, contractility recovery, and cell survival. These effects were Akt-dependent. In addition, no synergistic effect was observed with the combined treatments of cooling and baicalein. Our data suggest that baicalein may serve as a novel adjunct therapeutic strategy for SCA resuscitation. MDPI 2018-03-24 /pmc/articles/PMC5979521/ /pubmed/29587364 http://dx.doi.org/10.3390/ijms19040973 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shao, Zuohui
Chen, Sy-Jou
Zhu, Xiangdong
Lee, Chunpei
Huang, Hsien-Hao
Meliton, Angelo
Li, Changqing
Vanden Hoek, Terry L.
Li, Jing
Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
title Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
title_full Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
title_fullStr Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
title_full_unstemmed Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
title_short Baicalein Rescues Delayed Cooling via Preservation of Akt Activation and Akt-Mediated Phospholamban Phosphorylation
title_sort baicalein rescues delayed cooling via preservation of akt activation and akt-mediated phospholamban phosphorylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979521/
https://www.ncbi.nlm.nih.gov/pubmed/29587364
http://dx.doi.org/10.3390/ijms19040973
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