Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans

Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were...

Descripción completa

Detalles Bibliográficos
Autores principales: Renouard, Sullivan, Corbin, Cyrielle, Drouet, Samantha, Medvedec, Barbara, Doussot, Joël, Colas, Cyril, Maunit, Benoit, Bhambra, Avninder S., Gontier, Eric, Jullian, Nathalie, Mesnard, François, Boitel, Michèle, Abbasi, Bilal Haider, Arroo, Randolph R. J., Lainé, Eric, Hano, Christophe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979590/
https://www.ncbi.nlm.nih.gov/pubmed/29587452
http://dx.doi.org/10.3390/ijms19040990
_version_ 1783327733020884992
author Renouard, Sullivan
Corbin, Cyrielle
Drouet, Samantha
Medvedec, Barbara
Doussot, Joël
Colas, Cyril
Maunit, Benoit
Bhambra, Avninder S.
Gontier, Eric
Jullian, Nathalie
Mesnard, François
Boitel, Michèle
Abbasi, Bilal Haider
Arroo, Randolph R. J.
Lainé, Eric
Hano, Christophe
author_facet Renouard, Sullivan
Corbin, Cyrielle
Drouet, Samantha
Medvedec, Barbara
Doussot, Joël
Colas, Cyril
Maunit, Benoit
Bhambra, Avninder S.
Gontier, Eric
Jullian, Nathalie
Mesnard, François
Boitel, Michèle
Abbasi, Bilal Haider
Arroo, Randolph R. J.
Lainé, Eric
Hano, Christophe
author_sort Renouard, Sullivan
collection PubMed
description Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands.
format Online
Article
Text
id pubmed-5979590
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-59795902018-06-10 Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans Renouard, Sullivan Corbin, Cyrielle Drouet, Samantha Medvedec, Barbara Doussot, Joël Colas, Cyril Maunit, Benoit Bhambra, Avninder S. Gontier, Eric Jullian, Nathalie Mesnard, François Boitel, Michèle Abbasi, Bilal Haider Arroo, Randolph R. J. Lainé, Eric Hano, Christophe Int J Mol Sci Article Linum flavum hairy root lines were established from hypocotyl pieces using Agrobacterium rhizogenes strains LBA 9402 and ATCC 15834. Both strains were effective for transformation but induction of hairy root phenotype was more stable with strain ATCC 15834. Whereas similar accumulation patterns were observed in podophyllotoxin-related compounds (6-methoxy-podophyllotoxin, podophyllotoxin and deoxypodophyllotoxin), significant quantitative variations were noted between root lines. The influence of culture medium and various treatments (hormone, elicitation and precursor feeding) were evaluated. The highest accumulation was obtained in Gamborg B5 medium. Treatment with methyl jasmonate, and feeding using ferulic acid increased the accumulation of aryltetralin lignans. These results point to the use of hairy root culture lines of Linum flavum as potential sources for these valuable metabolites as an alternative, or as a complement to Podophyllum collected from wild stands. MDPI 2018-03-26 /pmc/articles/PMC5979590/ /pubmed/29587452 http://dx.doi.org/10.3390/ijms19040990 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Renouard, Sullivan
Corbin, Cyrielle
Drouet, Samantha
Medvedec, Barbara
Doussot, Joël
Colas, Cyril
Maunit, Benoit
Bhambra, Avninder S.
Gontier, Eric
Jullian, Nathalie
Mesnard, François
Boitel, Michèle
Abbasi, Bilal Haider
Arroo, Randolph R. J.
Lainé, Eric
Hano, Christophe
Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans
title Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans
title_full Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans
title_fullStr Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans
title_full_unstemmed Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans
title_short Investigation of Linum flavum (L.) Hairy Root Cultures for the Production of Anticancer Aryltetralin Lignans
title_sort investigation of linum flavum (l.) hairy root cultures for the production of anticancer aryltetralin lignans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5979590/
https://www.ncbi.nlm.nih.gov/pubmed/29587452
http://dx.doi.org/10.3390/ijms19040990
work_keys_str_mv AT renouardsullivan investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT corbincyrielle investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT drouetsamantha investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT medvedecbarbara investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT doussotjoel investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT colascyril investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT maunitbenoit investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT bhambraavninders investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT gontiereric investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT julliannathalie investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT mesnardfrancois investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT boitelmichele investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT abbasibilalhaider investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT arroorandolphrj investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT laineeric investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans
AT hanochristophe investigationoflinumflavumlhairyrootculturesfortheproductionofanticanceraryltetralinlignans

Ejemplares similares